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Montage of unmixed images for NLS (top) and SSASU (bottom). Panels A-P show the unmixed channels for autofluorescence (A, I); S. mitis/DY-415 (B, J); S. salivarius/DY-490 (C, K); Prevotella/ATTO 520 (D, L); Veillonella/ATTO 550 (E, M); Actinomyces/Texas Red-X (F, N); Neisseriaceae/ATTO 620 (G, O); and Rothia/ATTO 655 (H, P). A larger composite view of the non-autofluorescence unmixed channels is shown for NLS in panel Q and for SSASU in panel R. The scale bar in panel R indicates 10 µm.
Source publication
Spectral unmixing methods attempt to determine the concentrations of different fluorophores present at each pixel location in an image by analyzing a set of measured emission spectra. Unmixing algorithms have shown great promise for applications where samples contain many fluorescent labels; however, existing methods perform poorly when confronted...
Contexts in source publication
Context 1
... performance illustrated by these metrics can be ob- served qualitatively in the unmixing results for test image E2 (see Figure 3). In the results for NLS (Figure 3 A-H), autofluo- rescence has contaminated nearly all of the unmixed channels. ...
Context 2
... performance illustrated by these metrics can be ob- served qualitatively in the unmixing results for test image E2 (see Figure 3). In the results for NLS (Figure 3 A-H), autofluo- rescence has contaminated nearly all of the unmixed channels. This same autofluorescence light was efficiently captured in the autofluorescence channel of the SSASU unmixed image (Figure 3 I). ...
Context 3
... the results for NLS (Figure 3 A-H), autofluo- rescence has contaminated nearly all of the unmixed channels. This same autofluorescence light was efficiently captured in the autofluorescence channel of the SSASU unmixed image (Figure 3 I). We also note that Prevotella (ATTO 520) was not present in image E2, yet the ATTO 520 channel of the NLS unmixed image contained a significant amount of light (Figure 3 D). ...
Context 4
... same autofluorescence light was efficiently captured in the autofluorescence channel of the SSASU unmixed image (Figure 3 I). We also note that Prevotella (ATTO 520) was not present in image E2, yet the ATTO 520 channel of the NLS unmixed image contained a significant amount of light (Figure 3 D). The composite view of all unmixed fluorophore channels (i.e. ...
