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Minimum and maximum values, mean, and standard deviation of the inhibition zone diameters, epidemiological cutoff values, and wild type/non-wild type (WT/NWT) percentual for Lactococcus spp. and quality control strains in the antimicrobial susceptibility analysis.
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Lactococcus spp. has emerged as a pathogen that is affecting global aquaculture, with L. garvieae, L. petauri, and L. formosensis causing piscine lactococcosis. While antimicrobials are commonly used to treat diseases in aquaculture, reports of antimicrobial resistance in fish isolates are increasing. However, little is known about the susceptibili...
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Context 1
... isolates were then classified as wild-type (WT) or non-wild-type (NWT) [29]. To meet the minimum requirements of the NRI method [30], the disk diffusion data of the L. garvieae strains isolated from O. niloticus (n = 3), Trichogaster lalius (n = 1), and Xiphophorus maculatus (n = 1) from the Laboratory of Aquatic Animal Diseases culture collection were included in the calculation of the pECVs (Table S2). ...Context 2
... reference strains E. coli ATCC 25922 and Aeromonas salmonicida subsp. salmonicida ATCC 33658 presented inhibition zone diameters within the acceptable ranges established by the CLSI (Table 2). ...Context 3
... disk diffusion assay for L. formosensis strains exhibited zones ranging between 6 mm and 30 mm (Tables 2 and S1). All the isolates were categorized as NS (no observation of inhibition zone) for trimethoprim/sulfamethoxazole and norfloxacin (Figure 1). ...Context 4
... disk diffusion assay for L. garvieae strains exhibited zones ranging between 6 mm and 31 mm (Tables 2 and S1). The distribution of the inhibition zones obtained from all the evaluated isolates are shown in Figure S2. ...Context 5
... distribution of the inhibition zones obtained from all the evaluated isolates are shown in Figure S2. The calculated pECVs for the antimicrobials are presented in Table 2. A total of 8 and 15 isolates (Figure 1) presented a zone of complete inhibition of 6 mm for norfloxacin and trimethoprim/sulfametoxazole, respectively, preventing the establishment of the ECV for these antimicrobials. ...Context 6
... disk diffusion assay for the L. petauri strains exhibited zones ranging between 6 mm and 31 mm (Tables 2 and S1). All the isolates were classified as WT for neomycin. ...Context 7
... Materials: The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/microorganisms12112327/s1, Figure S1: Alluvial plot demonstrating the association of the host with antimicrobial susceptibility or resistance to florfenicol (FLO) and oxytetracycline (OXY) in L. formosensis (A), L. garvieae (B), and L. petauri (C) strains; Figure S2: Histograms of the inhibition zone for Lactococcus garvieae strains against amoxicillin (AMO), erythromycin (ERY), florfenicol (FLO), neomycin (NEO), norfloxacin (NOR), oxytetracycline (OXY), and trimethoprim/sulfamethoxazole (SXT); Table S1: Inhibition zones diameters (mm) of antimicrobial agents against Lactococcus spp. strains determined using disk diffusion susceptibility assay and MAR index calculated per isolate; Table S2: Inhibition zones diameters (mm) of antimicrobial agents against Lactococcus garvieae strains used to satisfy the minimum requirements of the NRI method. ...