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Microwave-assisted hydrodistillation (MAH) system adapted for the extraction of essential oils (EOs).
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Fusarium wilt, a vascular syndrome in a wide range of plants, is caused by the pathogen Fusarium Oxysporum. The objective of this investigation was to evaluate the antifungal effect of four essential oils (EOs) (Plectranthus amboinicus, Syzygium aromaticum, Lippia alba, and Rosmarinus officinalis), which were obtained by using microwave-assisted hy...
Citations
... (Antonio- Gutiérrez et al. 2023). ...
This work focuses on creating tiny oil droplets (nano-emulsions) from a cheap sugarcane bagasse residue to deliver the active ingredients in a plant extract (Plectranthus amboinicus) for use in medicine and food. Instead of expensive synthetic materials, scientists have proposed using a special type of fat (cell-stearate) made from recycled plant waste for this purpose. The extract's main components successfully produced nano-emulsions (NEMs) around 27-59 nm in size. The oil emulsifier (cell-stearate) has specific properties that keep the oil droplets separate and prevent them from clumping together. This is confirmed by measurements showing how the stabilizer changes the surface properties of the droplets, allowing them to mix better with water. The prepared nano-emulsion was in nano-range between 27.23 and 59.29 nm. The degree of substitution (DS) and HLB of cell-stearate was 0.17 and 7.26. The cell-stearate provided a protective layer around the extract droplet surface, which prevented droplets from coalescing. This hypothesis is supported by the contact angle measurement for the O/W emulsion formed by cell-stearate being 57°, which is lower than the value for emulsions formed by the emulsion of the extract with water, 105°. This study demonstrates a cost-effective way to produce a natural emulsifier for tiny oil droplets containing valuable plant compounds, potentially leading to new applications for recycled cellulose materials.
... The (1R,7S, E)-7-Isopropyl-4,10dimethylenecyclodec-5-enol is an alkenol class compound and found in the Pterocarpus pedatus, tick berry, ginger, Schizonepetae spica, Plectranthus amboinicus essential oil and extract which serve as antifungal inhibitory against Fusarium oxysporum (Chen 2018;Liu et al. 2020;Mugao et al. 2020;Antonio-Gutiérrez et al. 2023). ...
Murrinie ED, Fairuzia F, Arni N, Alpandari H, Maharini I. 2024. The metabolomic fingerprinting of four duku (Lansium domesticum) cultivars from Central Java, Indonesia based on unique metabolites and prospects for future breeding. Biodiversitas 25: 3816-3839. This research aims to map the genetic identity of Central Java (Indonesia) duku cultivars, Sumber, Woro, Matesih, and Kalikajar, through a metabolomic analysis approach using the Gas Chromatography-Mass Spectrophotometer (GC-MS) method from duku fruit peel and flesh. Heatmap and principal component analysis (PCA) of volatile organic compound (VOC) metabolites identified from duku fruit peel grouped Sumber, Woro, and Matesih as one group, with Sumber and Woro being more similar, while Kalikajar was in a different group. The identified metabolites of duku fruit flesh show that Woro and Matesih cultivars are in the same cluster, followed by Kalikajar, which is closer to the Woro and Matesih metabolites. Sumber mostly has different metabolites. VOC metabolites successfully identified primary and secondary metabolites defined as common metabolites in four cultivars, which have a myriad of functions for plant growth and development, nutritional value, plant defense against biotic stress and environmental stress tolerance, and physiological plant phenotypes. The successful identification of these metabolites not only enhances our understanding of the duku cultivars but also reassures the accuracy and reliability of future breeding for superior duku plants that are resistant to biotic and abiotic stress based on the VOC metabolites in the Sumber, Woro, Matesih, and Kalikajar cultivars, particularly the unique metabolites that distinguish these cultivars.
... to Antonio-Gutiérrez et al. (2023). These monoterpene hydrocarbons are attributed to its biological activities, particularly its potent antifungal properties against Fusarium oxysporum, suggesting its potential as a control agent for this fungal species. ...
Objetivo: O objetivo deste estudo é investigar o potencial socioambiental e terapêutico de Plectranthus amboinicus, explorando suas propriedades fitoquímicas, microbiológicas, antioxidantes e citotóxicas, bem como suas possíveis aplicações em diversos campos. Método: A metodologia utilizada nesta pesquisa envolve uma revisão integrativa de caráter qualitativo e quantitativo. Foram efetuadas buscas de artigos nas bases de dados Medline e LILACS por meio da Biblioteca Virtual em Saúde. Ao todo, foram selecionados 9 artigos, publicados entre 2019 e 2024, para compor este estudo. Resultados e Discussão: Os resultados obtidos revelaram que Plectranthus amboinicus possui um perfil fitoquímico diversificado, demostrando atividades antimicrobianas, antifúngicas, antioxidantes e anticancerígenas significativas. Os estudos indicam que pode ser um agente antimicrobiano promissor para novos medicamentos e produtos de controle microbiano, mas mais pesquisas são necessárias para entender seus mecanismos de ação, segurança e eficácia em modelos in vivo. Implicações da Pesquisa: As implicações da pesquisa podem abranger o desenvolvimento de novos medicamentos antimicrobianos, produtos de controle microbiano, cosméticos com propriedades fotoprotetoras, e métodos sustentáveis para aumentar a produção de compostos bioativos em plantas medicinais. Originalidade/Valor: Este estudo contribui para a literatura ao oferecer uma análise abrangente e integrativa do potencial de Plectranthus amboinicus, destacando suas propriedades antimicrobianas, antifúngicas e antioxidantes. A relevância e o valor desta pesquisa são evidenciados por destacar seu potencial para novos medicamentos e produtos naturais, além de métodos sustentáveis para aumentar a produção de compostos bioativos em plantas medicinais.
... MAE warrants further investigation to elucidate the interactions between patchouli compounds and their biological activities (Mokgehle et al., 2021). It is crucial to understand how valuable compounds can be obtained from patchouli by Microwave-Assisted Hydrodistillation (Antonio-Gutiérrez et al., 2023). To accomplish this, it is necessary to review previous studies, analyze the healing properties of these compounds, compare extraction methods, and examine research trends. ...
... The antifungal activity of essential oils extracted from other plant species has been investigated in numerous studies [49,[70][71][72][73][74][75]. Different ways of applying essential oils to plants are also used, e.g., dipping the seeds in the tested essential oils for 24 h reduces the Fusarium wilt and seedling rot and improved the survival of pea plants [76]. ...
... The essential oils studied also significantly reduced the concentrations of fungal mycotoxins, zearalenone and group B trichothecenes, in wheat grains [71]. The inhibitory effect of The antifungal activity of essential oils extracted from other plant species has been investigated in numerous studies [49,[70][71][72][73][74][75]. Different ways of applying essential oils to plants are also used, e.g., dipping the seeds in the tested essential oils for 24 h reduces the Fusarium wilt and seedling rot and improved the survival of pea plants [76]. ...
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Abstract
Modern integrated farming systems encourage the search for alternative (non-chemical), highly effective methods of plant protection. In this study, the fungistatic effect of fennel essential oil (FEO) and hop essential oil (HEO) on fungal growth and their ability to treat Fusarium wilt was investigated. The study was conducted in vitro and in pot experiments. The severity of infection was assessed by disease index (DI), presence of Fusarium culmorum gDNA (qPCR) and anatomical analyses of infected plant tissue using an optical (OM) and scanning electron microscope (SEM). Laboratory analyses showed that FEO inhibits mycelial growth of Fusarium fungi (F. avenaceum, F. culmorum, F. equiseti, F. oxysporum, F. poae, F. solani, F. sporotrichioides, F. tricinctum), Botrytis cinerea and Cylindrocarpon destructans more effectively than HEO. FEO at a concentration of 2000 ppm completely inhibited the growth of F. culmorum, F. poae and B. cinerea. Both essential oils reduced the severity of Fusarium wilt caused by F. culmorum in pea plants (DI, OM, SEM). The qPCR shows that both essential oils are also able to reduce the synthesis of trichothecenes in the tissues of infected pea plants. The results of the study suggest that FEO and HEO represent a broad spectrum bio-fungicidal agent that can be applied directly to plants at a concentration of 500 ppm, greatly reducing the level of infection.
Introduction: Lippia alba is a wild semishrubby species that grows in countries like Ecuador, its extracts have significant bioactive compounds. However, the antioxidant and antimicrobial properties of L alba essential oil (EO) have not been given importance in this region. The present study aimed to evaluate the physicochemical
characteristics of the extracts and biological activities of L alba EO. Methods: A unifactorial design was used, and physicochemical variables (pH, density, and refractive index) and antioxidant and antimicrobial capacities were explored using standard methods. Fractions of L alba EO were analysed by gas chromatography-mass spectroscopy. Results: The density, pH, refractive index, and phenolic content of L alba EO were found to be 0.95 g/ml, 5.5, 1.51, and 918 mg/l, respectively. An antioxidant activity of 23.08 mg/l was observed by the 2,2′-azino-bis-(3-
ethylbenzothiazoline-6-sulphonic) method, while 14.81 mg/l was observed by the ferric reducing antioxidant power method. Essential oil of L alba has presented a greater antimicrobial activity against different Gram- negative than Gram-positive bacteria. Essential oil of L alba showed an inhibitory zone of 56.7 and 9.7 mm against Photobacterium damselae sub sp. piscicida DSM 22834 and Bacillus subtilis ATCC 6633, respectively.
Conclusions: The results of the present investigation clearly indicated that the EO of L alba has potential antioxidant
and antimicrobial activities in vitro analysis, which are important
indications that this extract can be applied in different industries,
such as pharmaceutical industry, food industry, and others
