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Micrograph shows Fcwf-4 cell culture infected with FCoV UPM11C/08 at 15 h pi. Virus particles are present at the extracellular compartment and exhibit spherical shaped with spike-like electron-dense envelope (arrow). Virus particles are closely apposed to the plasma membrane (arrowhead). Scale bar, 0.2 µm.

Micrograph shows Fcwf-4 cell culture infected with FCoV UPM11C/08 at 15 h pi. Virus particles are present at the extracellular compartment and exhibit spherical shaped with spike-like electron-dense envelope (arrow). Virus particles are closely apposed to the plasma membrane (arrowhead). Scale bar, 0.2 µm.

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About growth of FIPV in FcWf cell line under TEM

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... and by 48 h pi, the virus particles were found to be present at both intracellular and extracellular. In the extracellular compartment, the virus particles were spherical measuring between 55 and 69 nm in diameter, with spike-like electron-dense envelope. Numerous virus particles were closely apposed to the host cell plasma membrane ( Fig. 2). At the same time, virus particles were observed to penetrate the cell by invaginating the plasma membrane. It carried into the cytoplasm part of the cell membrane that it invaginated to form vesicles ( Fig. 3). Virus-like particles which morphologically resembled those of extracellular virus particles were present in vacuoles of ...

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