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Metabolomics analysis. Statistical chart of differential metabolite quantity (A). Quality control principal components analysis (PCA) diagram (B). Volcano plots of differential metabolites among TB150 versus ZQ150 (C). Variable Importance in Projection (VIP) of orthogonal partial least squares discriminant analysis (TB150 versus ZQ150) (D). Z-score plot of differential metabolites (E).

Metabolomics analysis. Statistical chart of differential metabolite quantity (A). Quality control principal components analysis (PCA) diagram (B). Volcano plots of differential metabolites among TB150 versus ZQ150 (C). Variable Importance in Projection (VIP) of orthogonal partial least squares discriminant analysis (TB150 versus ZQ150) (D). Z-score plot of differential metabolites (E).

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Picea neoveitchii Mast. is a rare and threatened species of evergreen coniferous tree in China, commonly facing issues such as damaged seeds, abnormal seed growth, and empty seed shells. These abnormalities vary by location; unfortunately, the reasons behind these inconsistencies are completely unknown. This study compared seeds from two 150-year-o...