FIGURE 4 - available via license: Creative Commons Attribution 4.0 International
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Meiotic cells in the two spiders, male. (A, C) H. lycosina. (B) L. grahami. (A, C) Cells from the two spiders in the pachytene stage of meiosis exhibited positive heteropycnotic sex chromosomes. (C) Diakinesis cells confirmed the number of bivalents and sex chromosomes in H. lycosina with 11 autosomal bivalents and X 1 X 2 . The arrow represents the sex chromosome. Bar = 10 μm.
Source publication
Background
Karyotype and genome size are critical genetic characteristics with significant value for cytogenetics, taxonomy, phylogenetics, evolution, and molecular biology. The Lycosidae family, known for its diverse spiders with varying ecological habits and behavioral traits, has seen limited exploration of its karyotype and genome size.
Method...
Contexts in source publication
Context 1
... best chromosome dispersion was achieved with 0.075 mol/L KCl solution for 1.5 h (Supplementary Figure S3C). In addition, we found that the best results were observed when samples were stained with 10% Giemsa solution for 20 min (Supplementary Figure S4B). Therefore, this optimized protocol was subsequently applied for chromosome preparation in other species. ...
Context 2
... largest and smallest chromosomes of To demonstrate the existence of sex chromosomes in the two spiders, we conducted meiotic observations on males from each species. The analysis showed that in the meiotic cells of both male species, the sex chromosomes could be readily discerned during the pachytene nucleus due to their extreme condensation condensation and positive heteropycnosis ( Figures 4A,B). Diakinesis cells confirmed the number of bivalents and sex chromosomes in H. lycosina with Frontiers in Genetics frontiersin.org11 ...
