Figure - available via license: Creative Commons Attribution 4.0 International
Content may be subject to copyright.
Mean number of leaves per shoot of Passiflora edulis 'UENF Rio Dourado' in 100% MSM, 50% MSM, and 25% MSM media in
Source publication
The germplasm of Passiflora L. is conserved through seed banks and field collections. Dormant seeds, seeds with low viability, and high-cost field collections make it difficult to maintain germplasm banks, and in vitro conservation can be a complementary alternative. The aim of this study was to investigate the survival of nodal segments of Passifl...
Contexts in source publication
Context 1
... addition, temperature affected the number of leaves from 150 days in the media with higher mineral salt concentrations (100% MSM and 50% MSM) ( Table 1). Analysis of the interaction between mineral salts and sucrose revealed a decrease in the number of leaves in the media containing 50% MSM and 25% MSM salts in combination with 30 g L -1 sucrose (Table 2). After 150 days of slow growth, many plants began to lose their leaves because of the natural process of leaf abscission. ...Context 2
... 150 days of slow growth, many plants began to lose their leaves because of the natural process of leaf abscission. With 10 g L -1 of sucrose, the highest number of leaves was observed in media with 50% and 25% MSM, whereas with 20 and 30 g L -1 of sucrose, the highest number was obtained with 100% MSM salts (Table 2). Means followed by the same letters do not differ from each other (Tukey's test, p ≤ 0.05). ...Similar publications
Treatment of industrial sewage results in the production of a solid residue, sewage sludge, whose rich organic matter and chemical content makes it useful in agriculture. However, some soil components can be potentially toxic to humans if they enter the food chain. The aim of this study was to analyze the accumulation of heavy metals in the leaves...
Citations
... Although in situ conservation is essential for preserving plant species and maintaining genetic variability, it poses risks, such as invasion by pests, diseases, and natural disasters. Thus, ex-situ conservation is indicated for the conservation (Generoso et al., 2023). Ex-situ conservation is achieved through seed banks, a relatively low-cost method for preserving the genetic diversity of many individuals. ...
... Ex-situ conservation is achieved through seed banks, a relatively low-cost method for preserving the genetic diversity of many individuals. An alternative to reduce labor and time spent is to induce slow plant growth to reduce the turnover frequency (Chappell et al., 2020;Generoso et al., 2023). Slow or minimum growth is a biotechnological tool for medium-term plant germplasm conservation under in vitro conditions, reduces the metabolic activity, i.e., the growth rate of in vitro cultures, by maintaining them on a modified growth medium or in altered culture conditions (Chauhan et al., 2019). ...
Objective: To obtain protocols for clonal micropropagation, crop planning, and in vitro conservation of Rhycholaelia digbyana (Lindley) Schltr. Methodology: The effects of the Kundson C basal medium and benzylaminopurine concentration were evaluated for clonal micropropagation. The treatment with the greatest number of shoots formed per apex was selected for crop planning. Experiments were conducted to determine the effect of basal medium Murashige and Skoog concentration at 2.2 gL-1 and 4.4 gL-1; sorbitol, mannitol, and sucrose at 1, 2, and 3% on slow growth. Results: The best treatment for clonal micropropagation and crop planning was identified as 21.60 gL-1 Knudson C with 8.80 µM benzylaminopurine. This treatment resulted in uniform-sized shoots produced. The multiplication process can yield 10,240 seedlings in 12 months. Slow growth was achieved using Murashige and Skoog basal media at 2.2 gL-1 with 1% mannitol. Implications: More experiments must be conducted to determine the best shoot induction conditions and improve resource efficiency. Conclusions: These findings represent the first report on micropropagation and ex-situ conservation to preserve germplasm for this species as an important resource for the floriculture industry.
