Figure 5 - available via license: Creative Commons Attribution 4.0 International
Content may be subject to copyright.
MTX treatment effects on the Notch2 ligand Jag1, Notch2, and the Notch target gene Hey1 mRNA expression and the role of Notch2 signalling in MTX-induced Hey1 induction in cultured rat bone marrow-derived endothelial cells (BMECs). Quantitative real-time PCR gene expression analyses of (A) Jag1, (B) Notch2, (C) Hey1, using RNA isolated from control and MTX-treated (10 µM for 24 h) BMECs. (D) Quantitative real time PCR for Hey1 in BMECs treated for 24 h with Saline+Control IgG, Saline+Anti-Notch2 antibody (ab), MTX+Control IgG, or with MTX+Anti-Notch2 antibody. * p < 0.05, ** p < 0.01, and **** p < 0.0001 compared to control IgG; and ### p < 0.001 compared between MTX treated groups. All results are shown as mean ± SEM from three independent experiments.
Source publication
Intensive cancer chemotherapy is well known to cause bone vasculature disfunction and damage, but the mechanism is poorly understood and there is a lack of treatment. Using a rat model of methotrexate (MTX) chemotherapy (five once-daily dosses at 0.75 mg/kg), this study investigated the roles of the Notch2 signalling pathway in MTX chemotherapy-ind...
