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List of specimens used in this study

List of specimens used in this study

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The Neotropical tribe Dorynotini is characterized by a conspicuous tubercle or spine adorning the elytra, which, along with a few other characters, has been used to differentiate its recognized five genera and two subgenera. However, relationships among these taxa and the evolutionary origin of the pronounced tubercle remain speculative. Here we pr...

Contexts in source publication

Context 1
... morphological and molecular datasets, we sam- pled 16 species of Dorynotini, including four of the five genera and both subgenera (Supporting Information, Table S1). We were unable to include the monotypic genus Heteronychocassis, from French Guiana. ...
Context 2
... sampled a broad selection of outgroup taxa be- cause the relationships among tribes of Cassidinae remain contentious (Borowiec, 1995;Hsiao & Windsor, 1999;Chaboo, 2007). We sampled 15 species repre- senting the tribes that had been recovered in past studies as closely related to Dorynotini, Cassidini Gyllenhal, 1813, Ischyrosonychini Chapuis, 1875and Mesomphaliini Chapuis, 1875(Borowiec, 1995Hsiao & Windsor, 1999;Chaboo, 2007;Lopez et al., 2017) (Table 1). Specimens for sequencing were obtained during fieldwork in Bolivia, Brazil, the Dominican Republic, French Guiana and Panama. ...
Context 3
... phylogenetically informative adult mor- phological characters were used to assess interspe- cific morphological differences and build a discrete data matrix (Supporting Information, Appendix S1 and Table S2). They include 85 external anatomical characters and four internal anatomical characters (Supporting Information, Appendix S1). ...
Context 4
... chain reactions (PCRs) consisted of the following cycling steps: initial denaturation for 4 min at 95-98 °C; 30-40 cycles of denaturation for 30 s at 95-98 °C, annealing for 30 s at different temperatures depending on the primer pair (see below), and exten- sion for 1-1.5 min at 72 °C; with a final extension for 5-10 min at 72 °C. The annealing temperatures for each gene fragment were as follows: 50-51 °C for CO1 (Baca GenBank accession numbers, specimen voucher num- bers and collection data are provided in the Table 1. ...

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