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Kinetics of color formation in avocado seed paste. (A) A visual change in color of the paste was observed with the time of incubation. Representative results of 3 independent experiments. (B) Spectrophotometric analysis at 35 min showed a λmax at 480 nm. (C) Absorption at 480 nm (mean ±SD of 3 replicates) and (D) ΔE increased with the time of incubation (mean of 2 independent experiments).

Kinetics of color formation in avocado seed paste. (A) A visual change in color of the paste was observed with the time of incubation. Representative results of 3 independent experiments. (B) Spectrophotometric analysis at 35 min showed a λmax at 480 nm. (C) Absorption at 480 nm (mean ±SD of 3 replicates) and (D) ΔE increased with the time of incubation (mean of 2 independent experiments).

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There is an increasing consumer demand for and scientific interest in new natural colorants. Avocado (Persea americana) seed when crushed with water develops an orange color (= 480 nm) in a time-dependent manner. Heat treatment of the seed prevented color development, whereas the addition of exogenous polyphenol oxidase (PPO), but not peroxidase re...

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Context 1
... kinetics of color formation in avocado seed were followed by measuring changes in visible absorbance intensity and by de- termining L * a * b * values and color difference (E, Figure 1). Figure 1A shows visually how the orange color intensity increased with time. ...
Context 2
... kinetics of color formation in avocado seed were followed by measuring changes in visible absorbance intensity and by de- termining L * a * b * values and color difference (E, Figure 1). Figure 1A shows visually how the orange color intensity increased with time. The λ max of the color was 480 nm ( Figure 1B) and absorbance increased over the time course of the experiment in an exponential fashion approaching an asymptote by 20 min ( Figure 1C). ...
Context 3
... 1A shows visually how the orange color intensity increased with time. The λ max of the color was 480 nm ( Figure 1B) and absorbance increased over the time course of the experiment in an exponential fashion approaching an asymptote by 20 min ( Figure 1C). A similar time-dependent increase in E values was observed ( Figure 1D). ...
Context 4
... 1A shows visually how the orange color intensity increased with time. The λ max of the color was 480 nm ( Figure 1B) and absorbance increased over the time course of the experiment in an exponential fashion approaching an asymptote by 20 min ( Figure 1C). A similar time-dependent increase in E values was observed ( Figure 1D). ...
Context 5
... λ max of the color was 480 nm ( Figure 1B) and absorbance increased over the time course of the experiment in an exponential fashion approaching an asymptote by 20 min ( Figure 1C). A similar time-dependent increase in E values was observed ( Figure 1D). Within 0.5 min there was noticeable dif- ference in color compared to water. ...

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... Similarly, avocado seed extracts were reported to reduce the viability of human cancer cell lines through in vitro trials [15]. Previously, in 2011, Dabas et al. studied the potential use of an avocado seed extract as a natural colorant, finding that the development of the orange color is dependent on a polyphenol oxidase reaction [25]. Later, in 2019, Hatzakis et al. isolated the major colored compound in AS extract using high-performance liquid chromatography and identified it as perseorangin, a yellow-orange solid containing glycosylated benzotropone, with a distinctive orange color when in solution [26]. ...
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