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Karyotype diagrams for the two spiders. (A) Female H. lycosina (2n = 26, 14 m + 12 sm). (B) Male H. lycosina (2n = 24, 10 m + 14 sm). (C) Female L. grahami (2n = 20, 20th). (D) Male L. grahami (2n = 18, 12th + 6t). Positive and negative values indicate short and long arms, respectively.
Source publication
Background
Karyotype and genome size are critical genetic characteristics with significant value for cytogenetics, taxonomy, phylogenetics, evolution, and molecular biology. The Lycosidae family, known for its diverse spiders with varying ecological habits and behavioral traits, has seen limited exploration of its karyotype and genome size.
Method...
Contexts in source publication
Context 1
... optimal metaphase morphology was achieved when colchicine was used at a concentration of 0.05% and a treatment duration of 2 h (Supplementary Figure S2C). The best chromosome dispersion was achieved with 0.075 mol/L KCl solution for 1.5 h (Supplementary Figure S3C). In addition, we found that the best results were observed when samples were stained with 10% Giemsa solution for 20 min (Supplementary Figure S4B). ...
Context 2
... of the arm ratio (the length of the long arm/short arm, AR) of female and male chromosomes revealed that the mean AR (1.78) and AR range (1.23-2.44) in males were greater than the mean AR (1.75) and AR range (1.25-2.42) of females (Supplementary Table S2)). To compare the length of each chromosome more intuitively, the long arm and short arm of the chromosomes from male and female spiders were presented as bar charts (Figures 3A,B). The largest chromosomes in females and males were submetacentric (sm) and metacentric chromosomes (m), with lengths of 9.32 μm and 10.63 μm, respectively. ...
Context 3
... (Supplementary Table S2). For a more direct comparison of the length of each chromosome, we generated bar graphs (Figures 3C,D) to represent the long and short arms of the chromosomes in male and female L. grahami. The largest and smallest chromosomes of To demonstrate the existence of sex chromosomes in the two spiders, we conducted meiotic observations on males from each species. ...
