K2 knockout leads to apoptosis of anchorage‐dependent cells. (A) Representative confocal images of control, K2KO (K2 knockout by CRISPR‐Cas9), and K2KO + hK2 (K2 knockout with re‐expression of human K2) mouse embryonic fibroblasts (MEFs) stained with Phalloidin. Note that the loss of K2 expression was accompanied by morphological changes (stretched to round) which were reversed by re‐expression of human K2. Scale bars are 20 μm. (B) Flow cytometry analysis of Annexin V expression by control, K2KD (K2 knockdown by siRNA) and K2KD + hK2 (K2 knockdown with re‐expression of human K2) MEFs in adherent or suspension cultures. Numbers represent the percentages of cells in the corresponding quadrants. (C) Quantification of Annexin V⁺ apoptotic cells in B. N = 4. All values are mean ± SEM. Statistical significance was determined by one‐way ANOVA.

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Jul 2022

Objective: It is unclear why adhesion-dependent cells such as epithelium undergo anoikis without anchorage, while adhesion-independent blood cells thrive in suspension. The adhesive machinery of these cells is similar, with the exception of Kindlin orthologs, Kindlin 2 (K2) and Kindlin 3 (K3). Here we address how Kindlins control cell survival and...