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Inhibitory effects of F-CSA on LPS-induced inflammatory cytokines. The mRNA expression of (A) IL-6, (B) IL-1β, and (C) TNF-α was determined using RT-qPCR and normalized to control. ### p < 0.001 compared to vehicle; ** p < 0.01, *** p < 0.001 compared to LPS alone.
Source publication
The interest in bioconversion through fermentation of sprouts produced in smart farms is increasing due to their potential health benefits. Codonopsis lanceolata (CL) is reported to alleviate inflammatory conditions, but much research is still needed to determine which types and parts of CL are most effective. This study investigated the anti-infla...
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Codonopsis radix is the dried root of C. pilosula (Franch.) Nannf., C. pilosula Nannf. var. modesta (Nannf.) L. T. Shen, or C. tangshen Oliv., constitutes a botanical medicine with a profound historical lineage. It encompasses an array of bioactive constituents, including polyacetylenes, phenylpropanoids, alkaloids, triterpenoids, and polysaccharid...
Citations
... Codonopsis pilosula, a Chinese herb with a long history (Dong et al., 2023), has gradually received extensive attention from the medical community (Guo et al., 2024b) because of its various pharmacological effects such as antioxidant, anticancer, and antiinflammatory (Tian and Tang, 2022). Recent studies have shown that Codonopsis pilosula and its active ingredients play critical roles in a variety of diseases (Guo et al., 2024b), such as the cardiovascular system Shin et al., 2019), nervous system , digestive system (He et al., 2022), and immune system (Choi et al., 2023). However, whether Codonopsis pilosula has an effect on Article strategy and flowchart. ...
Introduction
Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor of the head and neck, with poor prognosis for advanced patients, and there is an urgent need to find new treatment strategies. Codonopsis pilosula, a traditional Chinese medicinal herb, possesses various pharmacological activities, but its antitumor effects and mechanisms in LSCC are still unclear. The aim of this study was to systematically investigate the potential antitumor mechanism of Codonopsis pilosula in LSCC.
Methods
In this study, we screened the effective compounds and targets of Codonopsis pilosula by TCMSP, ETCM and BATMAN-TCM databases, and screened targets related to LSCC by combining DisGeNET, GeneCards database and Cytoscape software. KEGG pathway enrichment analysis was utilized to explore the related signaling pathways. The core targets were further screened based on TCGA and GEO database analysis, and molecular docking was carried out to predict their binding ability to effective compounds. The presence of key compounds was verified by LC-MS, the MAPK3 expression was detected by qPCR in LSCC tissues, and the effects of MAPK3 knockdown on proliferation, migration, invasion, cell cycle, and apoptosis of LSCC cells were evaluated by cellular function assays.
Results
In this study, 22 targets of Codonopsis pilosula that might regulate LSCC were screened based on network pharmacology. KEGG pathway enrichment analysis showed that Codonopsis pilosula-LSCC targets were mainly involved in HIF-1, TNF, IL-17 and FoxO signaling pathways. Based on TCGA and GEO database analysis, MAPK3 was identified as the core target of Codonopsis pilosula-LSCC. The molecular docking results showed that a variety of effective compounds from Codonopsis pilosula had strong binding abilities to MAPK3, among them, Caprylic Acid, Emodin and Luteolin have been confirmed by LC-MS. QPCR analysis indicated that MAPK3 was highly expressed in LSCC tissues. MAPK3 knockdown significantly inhibits LSCC cell proliferation, migration and invasion. It also suppresses LSCC cell growth by blocking the cell cycle and inducing apoptosis.
Conclusion
Codonopsis pilosula exerts antitumor effects in LSCC through the regulation of MAPK3 and multiple signaling pathways, providing a theoretical basis for its clinical application.
... The fermented C. lanceolata root exhibited an increase in seven phenolic compounds, the most prominent of which was gallic acid, which enhanced the protective effect against glutamate-exposed hippocampal HT22 cells (Jin Bae Weon et al., 2014). Similarly, fermented C. lanceolata sprout extracts increased the contents of total flavonoids, tannins, and polyphenols, showing higher anti-inflammatory activity than original C. lanceolata sprout extracts (Choi et al., 2023). Based on these studies, fermented C. lanceolata might be a more valuable source of nutraceuticals than standard C. lanceolata. ...
... Colon cancer (Cao Y. et al., 2019), gastric precancerous lesions (He et al., 2022), liver cancer (Liu Z. et al., 2022;Li N. et al., 2024), hepatitis (Zeng et al., 2023), and colitis (Li F. et al., 2024) are malignant tumors or inflammatory diseases of the digestive system, and CP plays an important role in preventing and treating these diseases play an important role. Interestingly, CP can also regulate the immune function of sepsis (Choi et al., 2023), rheumatoid arthritis (Wang Y.-J. et al., 2022), allergic asthma (Seo et al., 2019), and other immune system disorders (Zou et al., 2019), reduce inflammatory reactions, and control the development and symptoms of diseases. ...
... In LPS-induced RAW264.7 cells Codonopsis pilosula reduced the expression of TNF-α, IL-6 and IL-1β in a dosedependent manner. It also inhibited p-p38, p-ERK, and p-JNK in a dose-dependent manner, thus inhibiting the JNK signaling pathway to exert anti-inflammatory effects (Choi et al., 2023). ...
As a traditional Chinese medicinal herb with a long history, Codonopsis pilosula (CP) has attracted much attention from the medical community in recent years. This review summarizes the research progress of CP in the medical field in the past 5 years. By searching and analyzing the literature, and combining with Cytoscape software, we comprehensively examined the role and mechanism of action of CP in individual application, combination drug application, and the role and mechanism of action of codonopsis pilosula’s active ingredients in a variety of diseases. It also analyzes the medicinal use of CP and its application value in medicine. This review found that CP mainly manifests important roles in several diseases, such as cardiovascular system, nervous system, digestive system, immune system, etc., and regulates the development of many diseases mainly through the mechanisms of inflammation regulation, oxidative stress, immunomodulation and apoptosis. Its rich pharmacological activities and diverse medicinal effects endow CP with broad prospects and application values. This review provides valuable reference and guidance for the further development of CP in traditional Chinese medicine.
... These pathways are named after them. For example, the MAPK pathway using JNK is called the JNK pathway [43]. ERK is an important mediator in signal transduction in response to cellular stress and various tissue injuries [44]. ...
Cysticercus pisiformis is a kind of tapeworm larvae of Taenia pisiformis, which parasitizes the liver envelope, omentum, mesentery, and rectum of rodents such as rabbits. Cysteine protease inhibitors derived from helminth were immunoregulatory molecules of intermediate hosts and had an immunomodulatory function that regulates the production of inflammatory factors. Thus, in the present research, the recombinant Stefin of C. pisiformis was confirmed to have the potential to fight inflammation in LPS-Mediated RAW264.7 murine macrophages. CCK8 test showed that rCpStefin below 50 μg/mL concentration did not affect cellular viability. Moreover, the NO production level determined by the Griess test was decreased. In addition, the secretion levels of IL-1β, IL-6, and TNF-α as measured by ELISA were decreased. Furthermore, it exerted anti-inflammatory activity by decreasing the production of proinflammatory cytokines and proinflammatory mediators, including IL-1β, IL-6, TNF-α, iNOS, and COX-2 at the gene transcription level, as measured by qRT-PCR. Therefore, Type I cystatin derived from C. pisiformis suppresses the LPS-Mediated inflammatory response of the intermediate host and is a potential candidate for the treatment of inflammatory diseases.
... Despite the wide range of biomaterials investigated recently for controlling inflammation in sepsis, several obstacles persist in preclinical studies. Firstly, the models used for sepsis are too narrowly focused (Choi et al., 2023). Many studies rely on LPS-induced sepsis models and are limited to in vitro studies using monocytes and macrophages (Jin et al., 2019;Kong et al., 2019;Lee et al., 2020;Hemmingsen et al., 2021). ...
Sepsis is a common and life-threatening disorder with an alarmingly high mortality rate. Unfortunately, this rate has not decreased significantly over the last decade and the number of septic cases is increasing each year. Despite sepsis affecting millions of people annually, there is still not an established standard of care. The development of a therapy that targets the thromboinflammation characteristic of sepsis is imperative. Until recently, research has focused on uncovering individual pathways to target. As more of the pathophysiology of sepsis has become understood and more biomarkers uncovered, the interplay between endothelial cells, platelets, and leukocytes has emerged as a critical event. Therefore, a multi-targeted approach is clearly required for designing an effective treatment for sepsis. The versatility of biomaterials offers a promising solution in that they can be designed to target and affect multiple pathways and systems and safely inhibit excessive inflammation while maintaining hemostasis. Already, studies have demonstrated the ability of biomaterials to target different processes and stages in sepsis-induced inflammation and coagulopathy. Moreover, some biomaterials offer inherent anti-inflammatory and hemostatic qualities. This review aims to discuss the most recent advancements in biomaterial development designed to address inflammation, coagulopathy, and thromboinflammation.
