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Influences of whole blood storage at room temperature, 30 or 37 C on thromboxane B2, elastase and thrombin-antithrombin III release in plasma during whole blood storage. (a) Thromboxane B2, (b) elastase and (c) thrombin-antithrombin III. *p < 0.05 compared with the baseline (0 min).  

Influences of whole blood storage at room temperature, 30 or 37 C on thromboxane B2, elastase and thrombin-antithrombin III release in plasma during whole blood storage. (a) Thromboxane B2, (b) elastase and (c) thrombin-antithrombin III. *p < 0.05 compared with the baseline (0 min).  

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The use of unactivated blood for hemocompatibility testing is essential to obtain reliable results. Here, the authors study the influence of heparinized whole blood storage time and temperature on blood activation and evaluate the importance of initiating hemocompatibility tests within 4 h of blood collection. Blood from healthy volunteers was coll...

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... The antibiofilm activity and hemocompatibility studies of SPIONs require the assessment of these nanoparticles with biological materials, which are bacterial biofilms and blood cells (ex.: red blood cells and platelets), respectively [4,5,[9][10][11][12][13][14]. These analyses of SPIONs have allowed the development of their applications in materials science and biomedical engineering [15][16][17][18][19][20]. ...
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... For the experiment, several boundary conditions for success were defined: (i) experiments must conclude within a 4-h time frame from the moment of blood drawing from the animal, because of a decrease in blood vitality in vitro. 3,4 (ii) Red blood cell count must be relatively stable for the duration of the experiment, because a decline in red blood cell count alters the Xray attenuation coefficient of the free-flowing blood in the CT scans. This would complicate the comparisons of consecutive CT scans, because no additional contrast agent besides hemoglobin was used. ...
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... A 21-gauge needle is to be used and the hand of the donor must not be strangled for a long time, to minimize venostasis and activation of the coagulation cascade. Blood is very sensitive, so it is important to be tested as soon as possible, within 4 h of collection [46,47]. Blood must be collected in tubes with anticoagulant to prevent blood clotting [20]. ...
... Incubation times are usually recommended of approximately 60 min. However, at most 4 h of incubation are possible due to blood instability [46,47,56,57]. ...
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Hemocompatibility testing is essential for the safe use of medical devices that come into contact with blood. There are various evaluation methodologies. In vivo, ex vivo and in vitro systems can be used and different categories can be evaluated in different ways. This review deals with in vitro hemocompatibility testing mainly on the basis of ISO standard 10993-4 recommendations and possibly new research results. This is a summary of all tested categories, i.e. coagulation, hemolysis, hematology and activation of leukocytes and platelets and the complement system. The main principle of evaluation and the possibilities of testing using various methodologies are always described. In the next part, variants of the method of blood incubation with the tested medical device from the static system to the circulation are described. Circulation can be provided, for example, by means of the Chandler Loop or parallel-plate chambers.