Identification of cuproptosis-related DEGs and exploration of the relationship between each CRG in HCC based on the TCGA database. (a) Cuproptosis-related DEGs expression patterns between HCC and normal tissue. The color legend represents the log2 (FPKM) value. (b) Pearson’s correlation analysis of each CRG based on the HCC samples. (c) PPI network plot displayed the relationship between each CRG. Red and green nodes indicate up and downregulated genes, respectively. (d) The number of adjacent nodes between each CRG in the PPI network. ∗p<0.05, ∗∗p<0.01, ∗∗∗p<0.001.

Identification of cuproptosis-related DEGs and exploration of the relationship between each CRG in HCC based on the TCGA database. (a) Cuproptosis-related DEGs expression patterns between HCC and normal tissue. The color legend represents the log2 (FPKM) value. (b) Pearson’s correlation analysis of each CRG based on the HCC samples. (c) PPI network plot displayed the relationship between each CRG. Red and green nodes indicate up and downregulated genes, respectively. (d) The number of adjacent nodes between each CRG in the PPI network. ∗p<0.05, ∗∗p<0.01, ∗∗∗p<0.001.

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Background: Hepatocellular carcinoma (HCC) remains a challenging medical problem. Cuproptosis is a novel form of cell death that plays a crucial role in tumorigenesis, angiogenesis, and metastasis. However, it remains unclear whether cuproptosis-related genes (CRGs) influence the outcomes and immune microenvironment of HCC patients. Method: From...

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... An enzyme of the lipoic acid pathway, LIAS generates antioxidants in mitochondria [68]. LIPT1 and DLD also represent enzymes of the lipoic acid pathway and participate in protein lipoylation, required for activation of cuproptosis [69]. CTR1/SLC31A1 and ATP7A/B, the copper transporter, are required for intracellular transport of the element, the trigger of cytotoxicity [70]. ...
... The discovery of cuproptosis, a new form of cell death [21,146], uncovered a relatively new copper-associated mechanism of signaling and a new anti-cancer target. Several recent studies reported discovery of coppertargeted genes associated with cuproptosis, including FDX1, LIAS, LIPT1, DLD, DLAT, PDHA1/B, MTF1, GLS, CDKN2A, ATP7A/B, SLC31A1 [29,65,66,69,70,[105][106][107][108]. Differential expression of these genes in malignant and normal tissues has been shown, suggesting their involvement in the regulation of carcinogenesis. ...
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... Its primary role is to catalyze the combination of fatty acids with coenzyme A (CoA), a critical step in intracellular fatty acid metabolism [6]. LIPT2 exhibits broad distribution in the cytoplasm, with notable expression levels observed in the liver, muscle, and adipose tissues, as indicated by studies conducted by Tang et al. in 2023 and Bernardinelli et al. in 2017 [7,8]. LIPT2 plays a crucial role in governing fatty acid synthesis, oxidation, and storage, thereby contributing to the overall balance of fatty acid metabolism, as outlined by Habarou et al. in 2017 and Solmonson and DeBerardinis in 2018 [6,9]. ...
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... version 4.0 (Jia et al., 2023). Furthermore, to investigate the biological functional differences among the groups, Gene Set Variation Analysis (GSVA) enrichment analysis was performed using the GSVA R package (Tang et al., 2023). ...
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