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Figure 3 - Autophagy Stimulation as a Potential Strategy Against Intestinal Fibrosis

Figure 3. Human primary fibroblasts isolated from the healthy margin of intestinal carcinoma resections (n = 5) were treated with TGF-β (5 ng/ml), in the presence of Rapamycin (50 nM), Bafilomycin B1 (10 nM) or their vehicles, for 24 hours. (A) Representative Western blots and protein levels of Col1a1, P62, LC3-I/II and GAPDH; and (B) graphs showing Col1a1 and P62 mRNA expression (results normalized with β-actin and represented as fold induction vs. vehicle-treated cells). Bars in graphs represent mean±S.E.M. Significant differences vs. vehicle-treated fibroblasts are shown by *p < 0.05, and ***p < 0.001; as analyzed by ANOVA with a Newman-Keuls post hoc correction for multiple comparisons or a t-test when appropriate (Graph-Pad Software v6.0).
Human primary fibroblasts isolated from the healthy margin of intestinal carcinoma resections (n = 5) were treated with TGF-β (5 ng/ml), in the presence of Rapamycin (50 nM), Bafilomycin B1 (10 nM) or their vehicles, for 24 hours. (A) Representative Western blots and protein levels of Col1a1, P62, LC3-I/II and GAPDH; and (B) graphs showing Col1a1 and P62 mRNA expression (results normalized with β-actin and represented as fold induction vs. vehicle-treated cells). Bars in graphs represent mean±S.E.M. Significant differences vs. vehicle-treated fibroblasts are shown by *p < 0.05, and ***p < 0.001; as analyzed by ANOVA with a Newman-Keuls post hoc correction for multiple comparisons or a t-test when appropriate (Graph-Pad Software v6.0).
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