Hippo overexpression upregulates Akh expression level. (A) Relative abundances of transcripts encoding RNA‐binding proteins (top) are plotted according to their degrees of fold changes. (B) Akh mRNA pull‐down (left) and Western blot of Me31b‐GFP (right) using S2 cell lysates. Akh mRNA RT‐qPCR of me31b‐GFP immunopellets from lysates of S2 cells transfected with empty vector or a Hpo‐Myc‐expressing plasmid. N = 3, ***p < 0.001, **p < 0.01, *p < 0.05, NS, not significant from Student's t‐test. (C) Akh mRNA level in S2 cells transfected with plasmids expressing me31b‐GFP or Hpo‐Myc relative to empty vector control. N = 3, **p < 0.01, *p < 0.05 from Student's t‐test. (D) Akh mRNA level in fat bodies of third instar larvae of Dcg/+, Dcg > Hpo, Dcg > Hpo/Me31b RNAi, and Dcg > Hpo/Dcp2 RNAi animals. N = 3, **p < 0.01 from Student's t‐test.