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It is well known that various plants (whole or some parts) are of definite
and useful use for human benefit and well fare. One of these benefits is the ability of
many plant seeds, fruits and different parts of exerting antimicrobial activity. The aim
of theses uses is the treatment of various infectious diseases. This would be clearly
understood t...
Citations
... A study published by (Kumar, 2015) has linked the nutritious and healing substances of the honey to the plants visited by bees , because it is the raw materials of honey . Nigella sativa is known by other names , and its names varying among places (Sufya et al., 2014). Nigella sativa oil contains 100 healing components working together in a synergetic manner. ...
The current research aimed to investigate the antibacterial activity of two types of honey alone and in combination with black seed oil and compare their effects. The first type of honey, the mountain type, was obtained from some beehive of Sulaymaniyah city, while the second type (Lowlander) was obtained from beehive in Balad city of Salah Al-Deen. The bacterial species used were Staphylococcus aureus, Escherichia coli, Salmonella typhi and Proteus mirabilis categorized into clinical and environmental groups. Antibacterial activity of the honeys was assayed using the Disc diffusion method. Different concentrations were conducted ranged from 20-100%. Results had revealed that the environmental bacterial isolates were affected mostly toward both honey types. However, the mountainous honey showed inhibitory effect against the Gram negative isolates. Mixture of honey in general with black seed oil had significantly differed and at some points decreased the effect while the black seed alone showed effect toward the environmental isolates at the absolute concentration.
... [142]. This finding may be attributed to the synergistic effect between BC seeds and Fe 2 O 3 -ZrO 2 at which they possessed superb phytogenic properties [143,144]. In addition, the ability of Fe 2 O 3 -ZrO 2 /BC to penetrate into the cell membrane and destroy the protein and DNA [145]. ...
The importance of different polymorphic forms of iron oxide nanoparticles attracted a lot of attentions in various applications due to their unique electrical, optical and magnetic properties. Moreover, the excellent biocom-patibility, high surface area, spherical shape, tunable nanoscale size and the availability of synthesis route make them desirable in various biological and pharmaceutical applications. To this aim, in this review, different synthesis methods of iron oxide nanoparticles were discussed, also the main characterization techniques used for elucidation of the iron oxide nanoparticles were reviewed. The exploitation of iron oxide nanoparticles-based systems as anticancer, antiviral, antimicrobial agents and its involvement in drug delivery system were reviewed in details. Additionally, the influence of nanoparticles size and the reagent type and conditions utilized in synthesis and their pharmaceutical applications was highlighted.
... [142]. This finding may be attributed to the synergistic effect between BC seeds and Fe 2 O 3 -ZrO 2 at which they possessed superb phytogenic properties [143,144]. In addition, the ability of Fe 2 O 3 -ZrO 2 /BC to penetrate into the cell membrane and destroy the protein and DNA [145]. ...
The importance of different polymorphic forms of iron oxide nanoparticles attracted a lot of attentions in various
applications due to their unique electrical, optical and magnetic properties. Moreover, the excellent biocompatibility, high surface area, spherical shape, tunable nanoscale size and the availability of synthesis route make
them desirable in various biological and pharmaceutical applications. To this aim, in this review, different
synthesis methods of iron oxide nanoparticles were discussed, also the main characterization techniques used for
elucidation of the iron oxide nanoparticles were reviewed. The exploitation of iron oxide nanoparticles-based
systems as anticancer, antiviral, antimicrobial agents and its involvement in drug delivery system were
reviewed in details. Additionally, the influence of nanoparticles size and the reagent type and conditions utilized
in synthesis and their pharmaceutical applications was highlighted.
... Dengan mekanisme yang seperti ini, tentu akan mempengaruhi efektivitas kerja dari jintan hitam, karena fisiologi bakteri gram negatif dengan gram positif berbeda. 9,26 Bakteri gram negatif memiliki membran luar yang membuat dinding selnya sulit ditembus oleh zat antimikroba, membran luar ini akan bertindak sebagai penghalang permeabilitas yang akan menghambat akses senyawa jintan hitam (mengingat ukuran molekul). Di sisi lain, bakteri gram positif tidak memiliki membran luar dan hanya terdiri dari dinding sel peptidoglikan sehingga lebih rentan terhadap agen antimikroba. ...
... Di sisi lain, bakteri gram positif tidak memiliki membran luar dan hanya terdiri dari dinding sel peptidoglikan sehingga lebih rentan terhadap agen antimikroba. 9,26 Hal ini sejalan dengan hasil yang didapatkan, dimana ekstrak jintan hitam pada kain lebih efektif membunuh bakteri gram positif (S. aureus) dibandingkan bakteri gram negatif (E.coli). ...
... Many kinds of research have been conducted to explore the pharmacological activities of the seeds and also the oil. The results showed that black seed oil has anti-inflammatory, anticancer, analgesic, antihypertensive, antimicrobial, antifungal, anthelmintic, hepatoprotective, diuretic, bronchodilator, gastroprotective and antidiabetic activities [2,3]. ...
Aims: The aims of this research were to develop and characterize liquid and solid micro emulsifying drug delivery system (L-SMEDDS and S-SMEDDS) containing black seed oil. Study Design: Experimental Research Design (laboratory). Place and Duration of Study: The study was conducted at research laboratory of pharmacy department UNISBA, between August 2018- August 2019. Methodology: The optimization of L-SMEDDS was carried out using various comparison of oil, surfactant, and cosurfactant. All formulations were evaluated for percent transmittance, emulsification time, dispersibility, robustness, and thermodynamic stability. The best formula of L-SMEDSS was evaluated for globule size distribution and converted to S-SMEDDS by spray drying method using aerosil 200 as adsorbent. S-SMEDDS were evaluated for organoleptic, flowability, compressibility, emulsification time, dispersibility, robustness and surface morphology. Results: The best formula of L-SMEDDS contains tween 80 as a surfactant and PEG 400 as cosurfactant (2:1) with a ratio of oil and Smix (2:8). The L-SMEDDS preparation meets the requirement of percent transmittance (95.77%), emulsification time (37.67 seconds), grade A of dispersibility, stable of robustness and thermodynamics study with the average of globule size was 231 nm. S-SMEDDS preparation meets the requirement of the moisture content, flowability, emulsification time, and stable on robustness testing with a spherical shape. Conclusion: L-SMEDDS and S-SMEDDS of black seed oil have been developed and have good physical characteristics and stability.
... A number of phytochemical and pharmacological studies have been conducted on Nigella sativa seeds because of its important biological activities. Also, the antimicrobial actions of fixed and volatile oils of various plant extracts have been completely investigated its prospect antibacterial action toward a wide range of bacterial species which collected from diarrheal stool samples [4]. Therefore, the producing of new antibiotics that is expensive and a consuming process, and pathogens are able to develop a rapid antibiotic resistance. ...
... For instance, in the current results, a crude oil of Nigella sativa showed antimicrobial effect against S. aureus; whereas, both Curcuma longa L and Zingiber officinale extracted oils showed a very lower inhibitory effect in comparison with Nigella sativa extracted oil (Figure 1). These findings are in agreement with other researchers who have showed that volatile oil of Nigella sativa shows the inhibitory action against antibiotic-resistant S. aureus [4,48]. It may be regarding to that S. aureus ATCC 25923 are more sensitive to antibacterial effect of extracted oils in comparison to Gram-negative E. coli, this controversy result may be due to the difference in their cell wall structure [49]. ...
Antibiotic resistance phenomena among pathogenic bacteria considered as a major health problem and associated with increased mortality or long-term hospitalization, which lead to open a new era by using plant and herbal extracts as an alternative source of various chemotherapeutic drugs, also to increase antibiotic efficiency by combining with plant extract for obtaining a powerful and broad spectrum action. The current investigation aims to investigate antibacterial actions of fixed oils of (Olea europaea L., Ricinus communis L. and Linum usitatissimum) and volatile oil of (Nigella sativa, Curcuma longa L and Zingiber officinale) against both Staphylococcus aureus strain (6734151) and Escherichia coli strain (5344572). This study conducted on antibacterial effect of six different extracted oils from medical herbs. The findings revealed that the oil extracts have different antibacterial activities with efficacy. Bacterial inhibition zone was detected by using disk diffusion method. Furthermore, volatile oil of N. sativa showed a great inhibitory action against resistant S. aureus, which was (27.7± 1.2 mm). The antimicrobial effects of other fixed and volatile oils against S. aureu, the inhibition zone was (10 ± 1.0 mm) for (Zingiber officinale), (9 ± 1.0 mm) for Ricinus communis L., (7.7 ± 0.6 mm) for Olea europea L., (7.3 ± 0.6 mm) for Linum usitatissimum and for Curcuma longa L. was (6.7 ± 0.6mm). Moreover, antimicrobial effect of N. sativa against E. coli was more active in comparison with other oils, while other oils showed a slight antibacterial effect. In conclusion, volatile oil of N. sativa reveals great antibacterial activities in comparison with other extracted oils.
... A number of phytochemical and pharmacological studies have been conducted on Nigella sativa seeds because of its important biological activities. Also, the antimicrobial actions of fixed and volatile oils of various plant extracts have been completely investigated its prospect antibacterial action toward a wide range of bacterial species which collected from diarrheal stool samples [4]. Therefore, the producing of new antibiotics that is expensive and a consuming process, and pathogens are able to develop a rapid antibiotic resistance. ...
... For instance, in the current results, a crude oil of Nigella sativa showed antimicrobial effect against S. aureus; whereas, both Curcuma longa L and Zingiber officinale extracted oils showed a very lower inhibitory effect in comparison with Nigella sativa extracted oil (Figure 1). These findings are in agreement with other researchers who have showed that volatile oil of Nigella sativa shows the inhibitory action against antibiotic-resistant S. aureus [4,48]. It may be regarding to that S. aureus ATCC 25923 are more sensitive to antibacterial effect of extracted oils in comparison to Gram-negative E. coli, this controversy result may be due to the difference in their cell wall structure [49]. ...
Antibiotic resistance phenomena among pathogenic bacteria considered as a major health problem ?and associated with increased mortality or long-term hospitalization, which lead to open a new era by using ?plant and herbal extracts as an alternative source of various chemotherapeutic drugs, also to increase antibiotic efficiency by combining with plant extract for obtaining a powerful and broad ?spectrum action. The current investigation aims to investigate antibacterial actions of fixed oils of (Olea europaea L., ?Ricinus communis L. and Linum usitatissimum) and volatile oil of (Nigella sativa, Curcuma longa L and ?Zingiber officinale) against both Staphylococcus aureus strain (6734151) and Escherichia coli ?strain (5344572). This study conducted on antibacterial effect of six ?different extracted oils from medical herbs. The findings revealed that the oil extracts have different ?antibacterial activities with efficacy. Bacterial inhibition zone was ?detected by using ?disk diffusion method. Furthermore, volatile oil of N. sativa ?showed a great inhibitory action ?against ?resistant S. aureus, which was (27.7± 1.2 mm). ?The antimicrobial effects of other fixed and volatile oils ?against S. aureu, the inhibition zone was (10 ± 1.0 mm) for (Zingiber officinale), ? (9 ± 1.0 mm) for Ricinus communis L., (7.7 ???± 0.6 mm) for Olea europea L., (7.3 ± 0.6 mm) for Linum usitatissimum and for Curcuma ?longa L. was (6.7 ± 0.6mm). Moreover, antimicrobial effect of N. sativa against ? E. coli was more ??active in comparison with other oils, while other oils showed a slight antibacterial effect?. In conclusion, volatile oil of N. sativa reveals great antibacterial activities in comparison with other extracted oils.
... A study published by (Kumar, 2015) has linked the nutritious and healing substances of the honey to the plants visited by bees , because it is the raw materials of honey . Nigella sativa is known by other names , and its names varying among places (Sufya et al., 2014). Nigella sativa oil contains 100 healing components working together in a synergetic manner. ...
The current research aimed to investigate the antibacterial activity of two types of honey alone and in combination with black seed oil and compare their effects. The first type of honey, the mountain type, was obtained from some beehive of Sulaymaniyah city, while the second type (Lowlander) was obtained from beehive in Balad city of Salah Al-Deen.
The bacterial species used were Staphylococcus aureus, Escherichia coli, Salmonella typhi and Proteus mirabilis categorized into clinical and environmental groups. Antibacterial activity of the honeys was assayed using the Disc diffusion method. Different concentrations were conducted ranged from 20-100%. Results had revealed that the environmental bacterial isolates were affected mostly toward both honey types. However, the mountainous honey showed inhibitory effect against the Gram negative isolates. Mixture of honey in general with black seed oil had significantly differed and at some points decreased the effect while the black seed alone showed effect toward the environmental isolates at the absolute concentration
... Thymohydroquinone has an antibacterial effect and decreases the number of bacterial colonies (Paarakh et al., 2010). Moreover, thymoquinone affects gramnegative bacteria by interfering with the bacterial envelope and impeding the bacterial cell from controlling its movement also causes bacterial lysis which leads to bacterial cell death (Ashraf et al., 2016;Sufya et al., 2014). The consumption of antioxidant obtained from N. sativa are found and can stimulate activities of phagocytic cells against Salmonella and regulate both the innate and acquired immune responses. ...
Nigella sativa extract (NSE) is known to be an effective antibacterial and immuno-modulating agent. This study aimed to demonstrate that treatment of Salmonella eterica subsp. eterica serovars typhimurium-infected mice with NSE would cause an increase in T-regulatory (T-reg) and Th2 cells and a decrease in the number of spleen bacterial colonies. Mice were randomly divided into five groups [control (T0), mice infected with S. typhimurium only (T1), and mice infected with S. typhimurium and then treated with NSE 0.52, 5.2, or 52 mg/kg body weight NSE (N1, N2, and N3, respectively)]. Levels of T-reg and Th2 cells were determined via flow cytometry and the number of spleen bacterial colonies was determined by observation. The results showed that treatment with NSE 0.52, 5.2, and 52 mg/kg significantly increased the number of T-reg and Th2 cells (p
... Chemical analysis of black seed oil indicated it was 20% protein, 37% carbohydrate, and 37 % fats and oils (unsaturated fatty acid and volatile oil) in addition to minerals, this is in agreement with others[40], most of the pharmacological effects are due to quinine constituents of which Thymoquinone and melanin are the major components, this is in accordance with other workers[41,42]. Thymoquinone and other component of black seed oil were qualitatively estimated according toAljabre et al. 2005 [41]andHoughton et al. 1995[42]. ...
Objective: Increasing use of medicinal plants in the treatment of infectious diseases are due to the development of multi-antibiotics resistant microorganisms, and had alerted our interest in the examination of some natural products. This study was carried out to investigate the antimicrobial activity of Jordanian propolis, black seed oil (Nigella sativa) extract, alone or in combination against clinically isolated microorganisms (bacteria and fungi).
Methods: Jordanian propolis samples were collected. Aqueous and alcoholic extractions were done; black seed oil was extracted from Nigella sativa seeds. Seven clinical isolated microorganisms namely: Micrococcus luteus, Bacillus pumilus, Bordetella bronchisptica, Enterococcus fecalis, Bacillus
subtilis, and Staphylococcus aureus, and one yeast strain namely Candida albicans were used. The antimicrobial activity was investigated by agar diffusion technique and microplate dilution to determine the MIC.
Results: The results indicated that the alcoholic propolis extract showed higher antimicrobial activity than the aqueous propolis extract. The antimicrobial activity of black seed oil was significantly higher than that of the propolis. Mixing propolis with black seed oil showed synergism effects against some microorganisms as Enterococcus fecalis (24±1.1), Bordetella bronchisptica (20±0.9) and Candida albicans (40±2.3), and additive with others as Bacillus subtilis (28±1.8).
Conclusion: Black seed oil and propolis might be used as a potential source of safe and effective natural antimicrobial in pharmaceutical and food industries
