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Gr a phical abstr act of showing the inter actions between LasR − pr otease pr oducers and LasR − pr otease-deficient individuals with casein as the sole carbon source and showing that pr opha ge induction in both subpopulations promotes population collapse. Created with BioRender.com.
Source publication
Social cheating is the exploitation of public goods that are costly metabolites, like exoproteases. Exoprotease exploitation in Pseudomonas aeruginosa has been studied in reference strains. Experimental evolution with reference strains during continuous growth in casein has demonstrated that non-exoprotease producers that are lasR mutants are selec...
Context in source publication
Context 1
... is that their replication during the growth in casein imposes a metabolic burden that perhaps could decrease the production and/or export of exoproteases, since the cellular resources will be used for phage pr oduction, whic h tends to increase during the continuous growth passa ges. Pr opha ge induction also causes the lysis of their host. Fig. 5 r epr esents a model describing our ...
Citations
... Transferring into quartz cells, the samples were read at 595 nm. 19,20 Determination of alginate. ...
Objetive
To evaluate the inhibitory effect of Quorum Sensing-dependent virulence factors in Pseudomonas aeruginosa by 2 molecules; curcumin and 5-bromoindole-3-carboxyaldehyde.
Methods
For the tests, 3 strains of P. aeruginosa were used; Pa01, Pa14 and ATCC 27853. The strains were reseeded in LB medium boxes for 12 hours at 37°. ON cultures of each strain were inoculated in LB broth, grown for 24 hours at 37°. Subsequently, new cultures of the strains were inoculated at starting from the ON cultures with an initial O.D 600 nm of approximately 0.0125, the following concentrations of the molecules were evaluated with these cultures: curcumin (1 and 5 μg/ml) and 5-bromoindole-3-carboxyaldehyde (30, 50 and 100 μg/ml). The factors were read virulence with their respective wavelength; protease (595 nm), elastase (495 nm), pyocyanin (520 nm) and alginate (530 nm).
Results
5-bromoindole-3-carboxyaldehyde significantly inhibited pyocyanin production in Pa01 (92.5% at 100 μg/ml), elastase in Pa01 (79.85% at 100 μg/ml), protease in Pa01 (92.8% at 50 μg/ml), and alginate in ATCC 27853 (76.16% at 100 μg/ml). Curcumin showed substantial inhibition of pyocyanin in ATCC 27853 (100% at 5 μg/ml), elastase in Pa14 (56.19% at 5 μg/ml), protease in Pa01 (87.04% at 5 μg/ml), and alginate in Pa01 (48.26% at 5 μg/ml).
Conclusions
Curcumin and 5-bromoindole-3-carboxyaldehyde demonstrated effective inhibition of QS-dependent virulence factors in P. aeruginosa . These findings suggest the potential of these compounds as anti-virulence agents, providing a promising alternative to conventional antibiotics in combating P. aeruginosa infections.
Pseudomonas aerugoinosa is a bacterium that causes important nosocomial diseases with high mortality and is classified within the bacteria of the acronym ESKAPE. The virulence of Psuedomonas aeruginosa is mediated by Quorum sensing, a cellular communication system that allows it to modulate the expression of virulence factors such as elastase, protease, siderophores, biofilm, alginate among others, through the LasI / LasR, RhlI / RhlR and PQS systems. Quorum inhibition allows an antivirulence strategy, where it has been shown that molecules such as curmunin and carboxaldehydes have considerable activity for this purpose. In the present work, the ability to inhibit Quorum Sensing-dependent virulence factors in Pseudomonas aeruginosa was evaluated using curcumin and 5-bromoindole-3-carboxaldehyde. The strains evaluated are Pa01, Pa14 and ATCC 27853 at concentrations (1 and 5 µg/ml) for curcumin and (30, 50 and 100 µg/ml) for 5-bromoindole-3-carboxaldehyde. 5-Bromoindole-3-carboxaldehyde significantly inhibited the production of pyocyanin in Pa01 (92.5% at 100 µg/ml), elastase in Pa01 (79.85% at 100 µg/ml), protease in Pa01 (92.8% at 50 µg/ml) and alginate in ATCC 27853 (76.16% at 100 µg/ml). Curcumin showed substantial inhibition of pyocyanin in ATCC 27853 (100% at 5 µg/ml), elastase in Pa14 (56.19% at 5 µg/ml), protease in Pa01 (87.04% at 5 µg/ml) and alginate in Pa01 (48.26% at 5 µg/ml). These findings suggest the potential of these compounds as antivirulence agents, to combat P. aeruginosa infections.
