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Flow cytometry histogram output confirming the tetraploid status (peak 2) of an Acacia mearnsii leaf vegetative sample compared to the diploid control (peak 1)
Source publication
Acacia mearnsii (black wattle) is grown commercially in South Africa for its timber and bark. However, the invasive nature of the species has resulted in it being considered an alien invader and for this reason research has been aimed at producing a sterile triploid variety that would be highly desirable for the South African commercial forestry in...
Context in source publication
Context 1
... cytometry analysis was used to confirm the stability (Shao et al. 2003, Harbard et al. 2012) of all 10 previously identified tetraploid trees. All 10 samples per tree were of the same ploidy, confirming the stability of tetraploidy in the vegetative tissues ( Figure 2). Ovule size measurements were taken from three isolation bags per tree in order to ascertain the stability of the reproductive tissues in the tetraploid (4 x ) population. Comparisons were made between isolation bags within a tree, between trees and against the diploid (2 x ) control. When examining the trees within the 4 x population, some significant differences ( p 0.05) were noted between isolation bags (Table 1). However, within both the 2 x control and 4 x population there was a range in ovule size, with the 2 x ovules ranging from 96.2 to 105.7 m (average 102.3 m) and 4 x ovules ranging from 118.2 to 137.3 m (average 123.9 m). These measurements were in agreement with those previously recorded for A. mearnsii (Beck-Pay 2012). On average measurements taken from within the 4 x isolation bags were, however, significantly ( p 0.05) larger than the 2 x control. Trees 71 and 77 had significantly ( p 0.05) lower ovule sizes than the other tetraploid trees but their sizes were still significantly ( p 0.05) larger than the 2 x control. Thus the results confirmed the ploidy of the tetraploid population and stability of this population under examination. Fresh A. podalyriifolia pollen (0 weeks) was collected and sieved and an average polyad size of 53.83 m was recorded. This was not significantly ( p > 0.05) different from that of pollen that was killed at 80 °C (Figure 3). Polyad sizes after 1–5 weeks of storage were significantly larger ( p 0.05) (55.45 m to 58.80 m) than the control treatment (53.83 m). The results from this preliminary study confirmed that the integrity of the polyad structure did not desiccate and shrink over time and thus polyads need not be measured immediately after collection. In order to ascertain the stability of the A. mearnsii 4 x population, polyad size measurements were taken from 10 isolation bags per tree. Comparisons were made between isolation bags within a tree, between trees and against the 2 x control. When examining the trees within the 4 x population, some significant differences ( p 0.05) were noted between isolation bags (Table 2). Within both the 2 x control and 4 x population there was a range of polyad sizes, with 2 x polyads ranging from 38.8 to 41.7 m (average 40.7 m) and 4 x polyads ranging from 46.4 to 50.9 m (average 48.7 m), confirming results previously reported by ...
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Citations
... It is therefore fundamental to determine the trade-off between the commercial value and related environmental problems. To avoid the undesirable impacts without interfering with industry purposes, the implementation of sterile lineages of acacias is under investigation (Beck and Fossey 2007;Beck-Pay 2013). We also suggest that forest managers, industries or land owners that benefit from the cultivation of exotic acacias should be economically responsible for the problems derived from their plantations. ...
The above- and belowground impacts due to Acacia invasions have been described in detail over the last 25 years. Future research should focus on the earlyd etection and prevention of new Acacia introductions and on a cost-effective and sustainable management of the novel ecosystems resulting from invasions.
