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Figure. Growth patterns of Colo ��5 cells exposed to cytokinin fractions of some medicinal �asidiomycetes: a -control; bHericium coralloides; c -Lentinula edodes; d -Fomitopsis officinalis. Concentration of cytokinin fraction is � μg/ml; ×��
Source publication
Background:
The anticancer effects of phytohormones of cytokinin nature are similar to those of medicinal mushrooms, which are able to synthesize cytokinins in large amounts.
Aim:
To determine the antiproliferative effect of crude extracts and cytokinin fractions from the mycelial biomass of seven fungi species on colon cancer cells in vitro.
M...
Citations
Nature has bestowed us with an abundant reservoir of resources that besides having nutritional value, are prolific mines of bioactive constituents with a plethora of medicinal activities. Mushrooms have been used since centuries in traditional system of medicine for their purported health benefits including anticancer activities. Thorough research, spanning over centuries in Japan, China, Korea, and the USA, has established the unique properties of mushrooms and their extractives in the prevention and treatment of various types cancer. The aim of the review article is to provide a comprehensive overview of the existing literature highlighting the potential relationship between mushrooms and colorectal cancer. Different databases such as PubMed, Web of Science, Google Scholar, and ScienceDirect were searched and a total of 62 articles and two book chapters were reviewed, and data were extracted. Multiple studies have demonstrated that mushrooms exhibit anticancer activities, effectively reducing adverse side effects such as nausea, myelosuppression, anemia, and sleeplessness. Furthermore, they have been shown to mitigate drug resistance following chemotherapy and radiation therapy. Certain species such as Antrodia , Pleurotus , Ganoderma , Lentinula , Hericium , Cantharellus , Clitocybe , Coprinopsis , Trametes , Sparassis , Lactarius , and so on manifest anticancer activity in colon. The article can help improve the scientific understanding of the co‐relationship between mushrooms and colorectal cancer. This may help in advancing the research directions and integrating the mushroom‐based strategies into current treatment protocols of colorectal cancer.
The use in clinical and pharmaceutical practice of biological preparations obtained from natural raw materials, in particular from mushrooms, is of urgent importance. Currently, the cultivation technologies of medicinal mushrooms are widely used, the mycelium of which contains a significant amount of cytokinins – stimulators of proliferation and differentiation of various types of plant cells, which also exhibit an immunomodulatory and oncostatic effects in the organism of animals and humans. Therefore, the aim of this work was to determine the cytotoxic/cytostatic effect of cultivated mushrooms Flammulina velutipes, Cyclocybe aegerita and Hericium erinaceus. Purification of cytokinins was carried out in stages, using methods of centrifugation, fractionation with n-butanol, ion exchange chromatography on Dowex 50Wx8 columns and thin-layer chromatography on Silicagel 60 F254. Identification and quantification of cytokinins was performed by by liquid chromatography in the reverse phase of MS (Agilent 1200 LC, USA) on an Agilent Zorbax Eclipse XDB-C18 column. Cytotoxic/cytostatic screening was performed on HeLa cells derived from human cervical cancer tumors using the MTT assay and routine counting of the ratio of live to dead cells. The results of the research showed that the highest content of cytokinins per 1 g of dry weight was characteristic of cultivated mushrooms of the species H. erinaceus. Also, H. erinaceus has the highest toxicity against human cervical cancer cells of the HeLa line, while the mushrooms F. velutipes and C. aegerita showed a cytostatic (antiproliferative) effect.
