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Figure 5 - Molecular Study of SHV-11 and SHV-12 Genes among Klebsiella Pneumoniae Isolated from UTI Patients in Erbil City

Figure (5): Agarose gel electrophoresis (1.2% agarose for 60-90 minutes) of amplified PCR products of blaSHV-11 gene (Genomic DNA) of phenotypic ESBL negative isolates Lane L: 1500 bp DNA ladder Lane 7-46: Amplified blaSHV-11 of ESBL negative isolates Consistent with previous results by Younes, (2010) who demonstrated that blaSHV-11 was the most prevalent gene described in his study, blaSHV-11 was highly prevalent in the current investigation and among 25 phenotypic ESBL negative isolates 23 isolates possess blaSHV-11. Moreover, out of 21 isolates included in the study of Lee et al., (2006) seventeen isolates contain blaSHV11 alone and associated with blaSHV-12 in 4 isolates. The blaSHV-11 has been described 
Agarose gel electrophoresis (1.2% agarose for 60-90 minutes) of amplified PCR products of blaSHV-11 gene (Genomic DNA) of phenotypic ESBL negative isolates Lane L: 1500 bp DNA ladder Lane 7-46: Amplified blaSHV-11 of ESBL negative isolates Consistent with previous results by Younes, (2010) who demonstrated that blaSHV-11 was the most prevalent gene described in his study, blaSHV-11 was highly prevalent in the current investigation and among 25 phenotypic ESBL negative isolates 23 isolates possess blaSHV-11. Moreover, out of 21 isolates included in the study of Lee et al., (2006) seventeen isolates contain blaSHV11 alone and associated with blaSHV-12 in 4 isolates. The blaSHV-11 has been described 
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