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Agarose gel electrophoresis (1.2% agarose for 60-90 minutes) of amplified PCR products of blaSHV-11 gene (Genomic DNA) of phenotypic ESBL positive isolates Lane L: 1500 bp DNA ladder 

Agarose gel electrophoresis (1.2% agarose for 60-90 minutes) of amplified PCR products of blaSHV-11 gene (Genomic DNA) of phenotypic ESBL positive isolates Lane L: 1500 bp DNA ladder 

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... It was shown that ESBL gene bla SHV-12 expressed~4-fold higher in the MDR strain of ST395 K47 than bla SHV -type genes coding broad-spectrum and narrowspectrum beta-lactamases in the remaining K. pneumoniae strains. This is in agreement with previously reported data that ESBL variants of the bla SHV-12 gene expressed higher than non-ESBL variants [46]. These genes did not change their expression after 90 min growing at 100 mg/L AMP or 10 mg/L CRO. ...
Early Response of Antimicrobial Resistance and Virulence Genes Expression in Classical, Hypervirulent, and Hybrid hvKp-MDR Klebsiella pneumoniae on Antimicrobial Stress
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  • Dec 2021
Klebsiella pneumoniae is an increasingly important hospital pathogen. Classical K. pneumoniae (cKp) and hypervirulent K. pneumoniae (hvKp) are two distinct evolutionary genetic lines. The recently ongoing evolution of K. pneumoniae resulted in the generation of hybrid hvKP-MDR strains. K. pneumoniae distinct isolates (n = 70) belonged to 20 sequence types with the prevalence of ST395 (27.1%), ST23 (18.6%), ST147 (15.7%), and ST86 (7.1%), and 17 capsular types with the predominance of K2 (31.4%), K57 (18.6%), K64 (10.0%), K1 (5.7%) were isolated from patients of the Moscow neurosurgery ICU in 2014–2019. The rate of multi-drug resistant (MDR) and carbapenem-resistant phenotypes were 84.3% and 45.7%, respectively. Whole-genome sequencing of five selected strains belonging to cKp (ST395K47 and ST147K64), hvKp (ST86K2), and hvKp-MDR (ST23K1 and ST23K57) revealed blaSHV, blaTEM, blaCTX, blaOXA-48, and blaNDM beta-lactamase genes; acr, oqx, kpn, kde, and kex efflux genes; and K. pneumoniae virulence genes. Selective pressure of 100 mg/L ampicillin or 10 mg/L ceftriaxone induced changes of expression levels for named genes in the strains belonging to cKp, hvKp, and hybrid hvKp-MDR. Obtained results seem to be important for epidemiologists and clinicians for enhancing knowledge about hospital pathogens.
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