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Agarose gel electrophoresis (1.2% agarose for 60-90 minutes) of amplified PCR products of blaSHV-11 gene (Genomic DNA) of phenotypic ESBL positive isolates Lane L: 1500 bp DNA ladder
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Seventy-five Klebsiella pneumonia isolates were identified according to their cultural characteristics and by Vitek2 system. Both genomic and plasmid DNA of the isolates were extracted. Polymerase Chain Reaction (PCR) was performed for detection of SHV-1gene in the plasmid DNA and SHV-12 gene in the genomic DNA of the isolates. SHV-1 found in the p...
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... et al., (2013) showed that the gene which was responsible for resistance to AMP reside in a 5000 bp mobile DNA segment which had the ability to translocate itself. The figure (4) shows the results of PCR amplification of blaSHV-11 of the phenotypic ESBL positive K. pneumoniae UTI isolates. After running of PCR products in agarose gel, the isolates which gave positive result in PCR, produced a band of 463 bp, which indicated the presence of blaSHV-11 gene. ...
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Seventy-five Klebsiella pneumonia isolates were identified according to their cultural characteristics and by Vitek2 system. Both genomic and plasmid DNA of the isolates were extracted. Polymerase Chain Reaction (PCR) was performed for detection of SHV-1gene in the plasmid DNA and SHV-12 gene in the genomic DNA of the isolates. SHV-1 found in the p...
Citations
... The non-ESBL phenotype conferred by SHV-11 shows that the Leu35Gln substitution between SHV-11 and SHV-1 has little or no significance with respect to hydrolysis of expanded-spectrum cephalosporins, therefore, its appearance is likely to be due to drift rather than antibiotic selection (Howard, et al. 2002). In contrast to the studies of Younes, (2010) and Abdullah (2013), blaSHV-1 is less prevalent in comparison to blaSHV-12. Their results demonstrated that blaSHV-1 was the most prevalent gene. ...
Seventy-five Klebsiella pneumonia isolates were identified according to their cultural characteristics and by Vitek2 system. Both genomic and plasmid DNA of the isolates were extracted. Polymerase Chain Reaction (PCR) was performed for detection of SHV-1gene in the plasmid DNA and SHV-12 gene in the genomic DNA of the isolates. SHV-1 found in the plasmid DNA of 28% of the isolates, while 52% of the isolates contained SHV-12 in their genomic DNA. Only 20% of the isolates carry both genes together
Klebsiella pneumoniae is an increasingly important hospital pathogen. Classical K. pneumoniae (cKp) and hypervirulent K. pneumoniae (hvKp) are two distinct evolutionary genetic lines. The recently ongoing evolution of K. pneumoniae resulted in the generation of hybrid hvKP-MDR strains. K. pneumoniae distinct isolates (n = 70) belonged to 20 sequence types with the prevalence of ST395 (27.1%), ST23 (18.6%), ST147 (15.7%), and ST86 (7.1%), and 17 capsular types with the predominance of K2 (31.4%), K57 (18.6%), K64 (10.0%), K1 (5.7%) were isolated from patients of the Moscow neurosurgery ICU in 2014–2019. The rate of multi-drug resistant (MDR) and carbapenem-resistant phenotypes were 84.3% and 45.7%, respectively. Whole-genome sequencing of five selected strains belonging to cKp (ST395K47 and ST147K64), hvKp (ST86K2), and hvKp-MDR (ST23K1 and ST23K57) revealed blaSHV, blaTEM, blaCTX, blaOXA-48, and blaNDM beta-lactamase genes; acr, oqx, kpn, kde, and kex efflux genes; and K. pneumoniae virulence genes. Selective pressure of 100 mg/L ampicillin or 10 mg/L ceftriaxone induced changes of expression levels for named genes in the strains belonging to cKp, hvKp, and hybrid hvKp-MDR. Obtained results seem to be important for epidemiologists and clinicians for enhancing knowledge about hospital pathogens.
