Figure 1 - available via license: Creative Commons Attribution 4.0 International
Content may be subject to copyright.
Source publication
Back ground: MRSA developed resistance to β-lactam antibiotics through the acquisition ofthe mecA gene that encodes penicillin-binding protein 2a (PBP2a), which has a significantlyreduced affinity for β-lactam antibiotics, thereby conferring β- lactam resistance . The detectionof mecA by the (PCR) is considered a gold-standard technique for methici...
Contexts in source publication
Context 1
... specimens included nasal swab, wound swab, burn swab, abscess and pus, sputum, ear swab, urine and blood culture. (150) isolates identified as Staphylococci on a mannitol salt agar depending on yellow color of the colonies, the media considered a selective and differential growth medium which is used for encouraging the growth of Staphylococci and inhibit others by containing high concentration of NaCl and phenol red as an indicator figure(1) (7).150 isolates gave a positive result and were identified as S. aureus due to the production of catalase enzyme which is distinguished them from Streptococcus spp. ...
Context 2
... the positive isolates (137) that were characterized as MRSA by the (methicillin disc test) were subjected to PCR to detect the presence of mecA gene, all of them gave positive results with 170pb PCR product figure(4). The acquisition of mecA gene is considered to be the first genetic requisite for methicillin resistance in Staphylococci (10 , 11 ,12). ...
