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Cromatograma obtenido del análisis por CG/EM de la fracción de hexano de las hojas de Clusia minor L. en el tiempo entre 8-26 min.
Source publication
"Gas Chromatography/Mass Spectrometry Characterization of the Apolar Extract from Clusia minor L. Leaves". Sterols, triterpenes, volatiles and other constituents in leaves of Clusia minor L. were analyzed by gas chromatography-mass spectrometry. Twenty five compounds were identified most of them new for the species. Sitosterol and stigmasterol were...
Context in source publication
Context 1
... partir de los 8 min y hasta los 30 min aproximadamente, pu- diendo ser identificados un total de 25 com- puestos. Todas las estructuras pudieron ser con- firmadas mediante una comparación espectral con las bases de datos Nist 98 y Wiley 275 al existir compuestos idénticos a los reportados en dichas bases. En la primera parte del cromato- grama ( Fig. 1), que comprende el tiempo entre 8-26 min, se observaron un grupo de compues- tos minoritarios que corresponden, en su mayo- ría, con sesquiterpenos y que constituyen el 21,7% de la fracción. Entre 27-30 minutos (Fig. 2) se pueden ob- servar un grupo de señales de gran intensidad (las más intensas) pertenecientes a los compues- tos ...
Citations
... Compounds 3, 7 were isolated from several species of Clusia (Da Silva Lins et al., 2016;De Andrade et al., 1998;Ferreira et al., 2015Ferreira et al., , 2016Mangas Marín et al., 2008;Oliveira et al., 2012;Teixeira et al., 2006), Mesua (Karunakaran et al., 2016a(Karunakaran et al., , 2016b, Garcinia (Elfita et al., 2009;Hartati et al., 2008;Mawa and Said, 2012;Momo et al., 2011;Ngoupayo et al., 2008;Ragasa et al., 2014;Rukachaisirikul et al., 2000;See et al., 2016See et al., , 2017Wong et al., 2017), Allanblackia (Nganou et al., 2018), and Pentadesma (Tala et al., 2013). Compounds 8 and 11 were isolated from C. melchiorii and C. obdeltifolia (Teixeira et al., 2006). ...
Some species of the Clusia genus have been shown to have important biomedical properties, including the ability to inhibit tumor growth in vitro and the usefulness for skin care. In this study, we examined the cytotoxic effect of hexane, ethyl acetate and methanol extracts from Clusia latipes Planch. & Triana, Clusiaceae, leaves on survival of human prostate cancer cells (PC-3), colon cancer cells (RKO), astrocytoma cells (D-384), and breast cancer cells (MCF-7). The ethyl acetate extract displayed the most substantial cytotoxic effect. However, using a Comet assay, we observed that the hexane extract induced a genotoxic effect (DNA damage) on human lymphocytes in an in vitro model. Chromatographic purification of the C. latipes hexane extract led to the isolation and identification of friedelin, friedolan-3-ol, and hesperidin as active cytotoxic compounds in hexane extract, while β-amyrine was identified as an active cytotoxic compound in the ethyl acetate extract of C. latipes, thereby supporting further studies of the molecular mechanisms underlying the effect of these secondary metabolites on cancer cell survival.
In the present work, the molecular composition of series of resinous aztec and maya archaeological samples were investigated to determinate their nature.Thus an analytical strategy was specifically designed. This analytical strategy included the analysis of botanically certified resins, freshly collected. It included as well the analysis of commercial samples bought in Mexican traditional markets.The study of all the samples included microscopical techniques, Fourier Inverse Infrared Spectrometry (FTIR) , High Performance Liquid Chromatography coupled to Ultraviolet-visible spectrometry (HPLC-UV/Vis) and Gas Chromatography coupled to Mass Spectrometry (GC-MS).The molecular study of these samples, in particular of their trterpenic composition allowed to : establish the molecular profile of resins from certified botanical origin. Identifiy some of the triterpenic compounds present in samples; Identify triterpenic molecules that could be uses as molecular markers for each botanical origin. Find the botanical origin of archeological astec samples. Discard possible botanic origins for maya archaeological sample. Have an overview of the origin of commercial samples of mexican copal. Create a simple protocol that allows conservation and biomaterials professionals to establish the botanical origin of archaeological and commercial resins, used in conservation interventions. Observe the behavior of copal materials upon ageing, establishing potential markers for natural ageing in copal, under darkness. Asses the botanical origin of a sample can be found regardless the age of the resin
