Fig5. SDS-PAGE of expresstion at different concentration of IPTG and tempreturewhere A (0.1 mM/37°C), B (0.1 mM/16°C), C (0.1 mM/37°C) and D (0.1 mM/1°C) M: protein marker, Lane BL21 free represents extract of -ve control. Lane PGEX free represents extract from expression cell containing PGEX plasmid, Lanes from 4 to 8: represent cell lysate of the strain harvested after 1, 2, 3, 4 and 24h.