Effect of sulforaphane on NF-κB expression. (A) NF-κB expression in neuro2a cell was measured by immunocytochemistry Left panel (FITC) is showing NF-κB positive cells, middle panel (DAPI) is showing total number of cells in the corresponding section and right panel is showing superimposed images. Increased green fluorescence indicates elevated NF-κB levels in neuro2a cells incubated with 30 mM glucose. This increase was abrogated by sulforaphane 10 µM. Next, we assessed the effect of sulforaphane on NF-κB in diabetic rats' sciatic nerve by western blotting (B) as well as by immunohistochemistry (C). The photographs were taken at 40 X. The arrowheads represent NFκB immunopositive nuclei. Both protein expression studies and immunohistochemical analysis of sciatic nerve micro-sections revealed that NF-κB expression was more pronounced in diabetic rats than in control rats which was brought down by treatment. Thus sulforaphane not only mitigates the oxidative damage occurring in hyperglycaemic conditions, but also dampens the inflammatory response mediated by NFκB . NG and HG: neuro2a cells incubated in 5.5 and 30 mM glucose respectively; HG+SFN: neuro2a cells treated with sulforaphane 10 µM in the presence 30 mM glucose; ND: non diabetic; STZ-D: Diabetic; D+SFN 0.5 and D+SFN 1: Diabetic group treated with sulforaphane 0.5 and 1 mg/kg respectively. Results are Mean ± SEM of three independent experiments. ### p<0.001 Vs ND; * p<0.05 and ***p<0.001Vs STZ-D.  

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... is proposed to down regulate the expression of NF- κB, hence levels of NF-κB were determined after activating Nrf2 with sulforaphane. Immunofluorescent analysis reveals that increase in levels of NF-κB was induced when neuro2a cells were incubated with 30 mM glucose which was counteracted by sulforaphane (Fig. 5A). A similar elevation in NF-κB was seen in sciatic nerve of untreated diabetic animals. In-vivo treatment with sulforaphane produced a significant dose dependant decrease in NF-κB expression (Fig. 5B). Findings of protein expression studies were perpetuated by immunohistochemical analysis of sciatic nerve micro-sections where the number ...

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... reveals that increase in levels of NF-κB was induced when neuro2a cells were incubated with 30 mM glucose which was counteracted by sulforaphane (Fig. 5A). A similar elevation in NF-κB was seen in sciatic nerve of untreated diabetic animals. In-vivo treatment with sulforaphane produced a significant dose dependant decrease in NF-κB expression (Fig. 5B). Findings of protein expression studies were perpetuated by immunohistochemical analysis of sciatic nerve micro-sections where the number of NF-κB (p65 subunit) immunopositive cells was more pronounced in diabetic rats than in control rats (p<0.001). Sulforaphane 0.5 mg/kg (p<0.05) and 1 mg/kg (p<0.001) produced a significantly reduced ...

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... by immunohistochemical analysis of sciatic nerve micro-sections where the number of NF-κB (p65 subunit) immunopositive cells was more pronounced in diabetic rats than in control rats (p<0.001). Sulforaphane 0.5 mg/kg (p<0.05) and 1 mg/kg (p<0.001) produced a significantly reduced NF-κB (p65 subunit) immunopositive cells in treated diabetic rats (Fig. ...