Fig 4 - uploaded by Ashutosh Kumar
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Effect of sulforaphane on HO-1and NQO-1 expression. HO-1and NQO-1 protein levels were measured by Western blotting in diabetic rats sciatic nerve (A) and in neuro2a cells (B) and equal loading was confirmed by β-Actin. Since HO-1and NQO-1 are under transcriptional regulation of Nrf2, their levels were also were analyzed in diabetic condition. Similar to Nrf2, these proteins were decreased significantly in diabetic rats and in neuro2a cells incubated in high glucose media. Treatment with sulforaphane demonstrated improved levels of HO-1 and NQO-1 in in vitro and in vivo studies. ND: non diabetic; STZ-D: Diabetic; D+SFN 0.5 and D+SFN 1: Diabetic group treated with sulforaphane 0.5 and 1 mg/kg respectively; NG and HG: neuro2a cells incubated in 5.5 and 30 mM glucose respectively; NG+SFN and HG+SFN: neuro2a cells treated with sulforaphane 10 µM in the presence of 5.5 and 30 mM glucose respectively. Results are Mean ± SEM of three independent experiments. (A) ## p<0.01 and ### p<0.001 Vs ND; * p<0.05 and ** p<0.01 Vs STZ-D. (B) ### p<0.001 Vs NG; *** p<0.001 Vs HG.
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