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Effect of sulforaphane on Nrf2 expression. Nrf2 protein levels were measured by Western blotting in diabetic rats sciatic nerve (A) and in neuro2a cells (B) and equal loading was confirmed by β-Actin. After eight weeks of diabetes in rats or after 24 h incubation of neuro2a cells with high glucose, there was a significant reduction in the Nrf2 protein levels. Both, in vitro and in vivo experiments demonstrated that sulforaphane increased the expression of Nrf2 thereby conferring protection against high glucose led oxidative stress. ND: non diabetic; STZ-D: Diabetic; D+SFN 0.5 and D+SFN 1: Diabetic group treated with sulforaphane 0.5 and 1 mg/kg respectively. Results are Mean ± SEM of three independent experiments. (A) ### p<0.001 Vs ND; * p<0.05 and ** p<0.01 Vs STZ-D. (B) # p<0.05 Vs NG; * p<0.05 and ** p<0.01 Vs HG.  

Effect of sulforaphane on Nrf2 expression. Nrf2 protein levels were measured by Western blotting in diabetic rats sciatic nerve (A) and in neuro2a cells (B) and equal loading was confirmed by β-Actin. After eight weeks of diabetes in rats or after 24 h incubation of neuro2a cells with high glucose, there was a significant reduction in the Nrf2 protein levels. Both, in vitro and in vivo experiments demonstrated that sulforaphane increased the expression of Nrf2 thereby conferring protection against high glucose led oxidative stress. ND: non diabetic; STZ-D: Diabetic; D+SFN 0.5 and D+SFN 1: Diabetic group treated with sulforaphane 0.5 and 1 mg/kg respectively. Results are Mean ± SEM of three independent experiments. (A) ### p<0.001 Vs ND; * p<0.05 and ** p<0.01 Vs STZ-D. (B) # p<0.05 Vs NG; * p<0.05 and ** p<0.01 Vs HG.  

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