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FIG 3 - Courtship Ritual of Male and Female Nuclei during Fertilization in Neurospora crassa

FIG 3 Female nuclear speeds before and after male nucleus entry into the trichogyne. Analysis of the time-lapse Movies S3a and b. (A) Plot; (B) numerical values. The speed of the green-labeled H1-sGFP female nuclei of the trichogyne and of the vegetative hyphae in the same field of view were measured at every time frame before (Movie S3a) and after (Movie S3b) entry of a male nucleus into the trichogyne. S.D, standard deviation; N nuclei , number of analyzed nuclei in the field of view; N speed , number of nuclei speeds measured. Results from the ANOVA (on log values) indicate that the effect of target site is significant (P , 0.001), with larger values in hyphae (1.56 6 1.63, n = 683) than in trichogyne (0.92 6 0.94, n = 749); likewise, the effect of time of observation is significant (P , 0.001), with larger values before (1.42 6 1.73, n = 569) than after (1.10 6 1.01, n = 863); these two effects are independent of one another as confirmed by the nonsignificant interaction (P = 0.431). Pairwise t tests with pooled SD indicate that all pairs of means were different at the 5% level.
Female nuclear speeds before and after male nucleus entry into the trichogyne. Analysis of the time-lapse Movies S3a and b. (A) Plot; (B) numerical values. The speed of the green-labeled H1-sGFP female nuclei of the trichogyne and of the vegetative hyphae in the same field of view were measured at every time frame before (Movie S3a) and after (Movie S3b) entry of a male nucleus into the trichogyne. S.D, standard deviation; N nuclei , number of analyzed nuclei in the field of view; N speed , number of nuclei speeds measured. Results from the ANOVA (on log values) indicate that the effect of target site is significant (P , 0.001), with larger values in hyphae (1.56 6 1.63, n = 683) than in trichogyne (0.92 6 0.94, n = 749); likewise, the effect of time of observation is significant (P , 0.001), with larger values before (1.42 6 1.73, n = 569) than after (1.10 6 1.01, n = 863); these two effects are independent of one another as confirmed by the nonsignificant interaction (P = 0.431). Pairwise t tests with pooled SD indicate that all pairs of means were different at the 5% level.
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