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- Extracellular Vesicle-Mediated Modulation of Stem-like Phenotype in Breast Cancer Cells under Fluid Shear Stress

Expression of stemness markers and microRNAs in serum-free MDA-MB-231 cells and corresponding EVs with and without FSS. (A) RNA levels of stemness-related genes NANOG and OCT4 normalized to ACTB. (B) miRNA levels in static- and FSS-cultured MDA-MB-231 cells and (C) in their corresponding EVs normalized to reference miRNA marker miR-30e. n = 3 biological replicates each with 2 or 3 technical replicates; mean ± standard error; ** p < 0.01; **** p < 0.0001; ns = not significant via Student’s t-test.
Expression of stemness markers and microRNAs in serum-free MDA-MB-231 cells and corresponding EVs with and without FSS. (A) RNA levels of stemness-related genes NANOG and OCT4 normalized to ACTB. (B) miRNA levels in static- and FSS-cultured MDA-MB-231 cells and (C) in their corresponding EVs normalized to reference miRNA marker miR-30e. n = 3 biological replicates each with 2 or 3 technical replicates; mean ± standard error; ** p < 0.01; **** p < 0.0001; ns = not significant via Student’s t-test.
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