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Experimental procedure; (a) culture plate of Ganoderma sessile used in the experiments, (b) sanitization of the model figure with sporicide and ethanol 70%, (c) close-up view of the myceliated model figure, (d) exemplar locations of electrodes on the living mycelium exoskin.
Source publication
Advancements in mycelium technology, stemming from fungal electronics and the development of living mycelium composites and skins, have opened new avenues in the fusion of biological and artificial systems. This paper explores an experimental endeavour that successfully incorporates living, self-regenerating, and reactive Ganoderma sessile mycelium...
Contexts in source publication
Context 1
... literature [25,12,11,23]. A living culture of G. sessile was provided by MOGU S.r.l. (Inarzo, Italy) with collection code 95-19 pv5. The mycelium was sub-cultured on Potato Dextrose Agar (VWR Chemicals, USA) in 90mm Petri dishes. To promote optimal growth, the culture was kept in darkness at an ambient room temperature of circa 22-23°C for 5 days (Fig. ...
Context 2
... stirring conditions, and homogenised with a laboratory blender before use (Waring, USA). The cyborg figure model, made from plastic resin, was subsequently prepared to accommodate the mycelium growth. The figure surface was pre-treated with a commercial sporicide (Ecolab, DE) and with ethanol 70%, and washed repeatedly with sterile demi-water (Fig. 2b). To allow the fungal tissue to grow on the surface, the cyborg model figure was coated with a thin layer of malt extract agar (MEA). Once the MEA coating gelatinized, the figure was briefly submerged in the homogenised liquid culture previously prepared to allow the fungal hyphae to attach. The model was then placed in a PP5 ...
Context 3
... activity was recorded using iridium-coated stainless steel subdermal needle electrodes (Spes Medica S.r.l., Italy). Four pairs of electrodes were inserted into the neck (channel 1-2), the back (channel 3-4), and the two arms (channel 5-6 in the right arm and channel 7-8 in the left arm) of the mycelium-covered model ( Fig. 2cd and Fig. 3) with distances between electrodes ranging of approx. 1 ...
