Estimation plots of chlorophyll a concentrations. Dots represent the biocrust 261 samples, and colors represent either dry or incubated soil. 262 263

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... p < 0.01; Table 1). Soil organic matter was also significantly 242 higher in the natural biocrusts (t = 3.77, p < 0.01; Table 1). pH was slightly higher in natural 243 biocrusts; however, the differences were not statistically significant (pH: t = 1.41, p = 0.19; 244 The estimation plot revealed an effect size estimate at 1.42 (95CI -0.432; 3.03; Fig. 3). In the 254 natural samples, there was no clear clustering according to the soil water content i.e., dry or 255 hydrated (following 96 hr incubation with water). In fact, there was a larger variance between 256 samples collected after incubation (Fig. 3). Hydrated post-mining biocrusts had consistently 257 higher chlorophyll a ...

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... p = 0.19; 244 The estimation plot revealed an effect size estimate at 1.42 (95CI -0.432; 3.03; Fig. 3). In the 254 natural samples, there was no clear clustering according to the soil water content i.e., dry or 255 hydrated (following 96 hr incubation with water). In fact, there was a larger variance between 256 samples collected after incubation (Fig. 3). Hydrated post-mining biocrusts had consistently 257 higher chlorophyll a concentrations compared to dry biocrusts. It Is also apparent that the 258 variance between samples was smaller in the post-mining biocrusts (Fig. 3). https://doi.org/10.5194/egusphere-2022-1510 Preprint. Discussion started: 9 January 2023 c Author(s) 2023. CC BY ...

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... (following 96 hr incubation with water). In fact, there was a larger variance between 256 samples collected after incubation (Fig. 3). Hydrated post-mining biocrusts had consistently 257 higher chlorophyll a concentrations compared to dry biocrusts. It Is also apparent that the 258 variance between samples was smaller in the post-mining biocrusts (Fig. 3). https://doi.org/10.5194/egusphere-2022-1510 Preprint. Discussion started: 9 January 2023 c Author(s) 2023. CC BY 4.0 License. (Table S2) (Table S3). Following decontamination and filtering, 86% of the ASVs were removed (Table 266 S3). However, they accounted for only 16% of the total reads. Out of the remaining 1,404 ...

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... incubation, most biocrust samples displayed some degree of greening, with more greening 366 in the natural biocrusts (Fig. 2). This indicates that the photosynthetic bacteria in the 367 biocrust were activated upon wetting. Yet, no significant differences were detected between 368 natural and post-mining biocrusts, chlorophyll a concentrations (Fig. 3) or functional 369 predictions (Fig. 6). This implies that similar abundances of photosynthetic bacteria were 370 activated upon wetting in both biocrusts, yet, they barely proliferated (Fig. 4). ...