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Figure 6 - General Patterns and Species-Specific Differences in the Organization of the Tubulin Cytoskeleton in Indeterminate Nodules of Three Legumes

Figure 6. Endoplasmic microtubule organization in infected cells of nitrogen fixation zone. (А,B) Vicia sativa L., (C,D) Galega orientalis Lam, and (E,F) Cicer arietinum L. Confocal laser scanning microscopy of 50 μm longitudinal vibratome sections. (A-F) Immunolocalization of tubulin (microtubules), green channel; DNA staining with propidium iodide (nuclei and bacteria), red channel. (A,C,E) Merged images of a single optical section of differential interference contrast and maximum intensity projection of optical sections in green channel. (B,D,F) Maximum intensity projections of 40 optical sections in green and red channels. n, nucleus; ic, infected cell; uic, uninfected cell. Bars, 10 μm.
Endoplasmic microtubule organization in infected cells of nitrogen fixation zone. (А,B) Vicia sativa L., (C,D) Galega orientalis Lam, and (E,F) Cicer arietinum L. Confocal laser scanning microscopy of 50 μm longitudinal vibratome sections. (A-F) Immunolocalization of tubulin (microtubules), green channel; DNA staining with propidium iodide (nuclei and bacteria), red channel. (A,C,E) Merged images of a single optical section of differential interference contrast and maximum intensity projection of optical sections in green channel. (B,D,F) Maximum intensity projections of 40 optical sections in green and red channels. n, nucleus; ic, infected cell; uic, uninfected cell. Bars, 10 μm.
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