Table 1 - uploaded by Eric Fossel
Content may be subject to copyright.
-Effect of transdermal L-arginine cream on temperature Metatarsal (°F) P vs. visit 1 Big toe (°F) P vs. visit 1
Context in source publication
Context 1
... subjects were enrolled, and 13 completed the study (aged 56 8 years). After analyzing the data, it was clear that the effect of L-arginine persisted throughout the washout periods (Tables 1 and 2). Because of this, except for the initial exposure of L-arginine virgin feet, the analysis was altered to determine the effect from cumulative exposure to L-arginine throughout the protocol. ...
Citations
... The dietary supplement L-arginine improved microperfusion of the rat skin (42). Consistently, transdermal delivery of L-arginine improved cutaneous blood flow in the feet of diabetic patients (43). Significant improvement of reddish coloration of the face under standardized testing conditions provided the first line of evidence suggesting that shilajit may improve skin microcirculation. ...
Objective: Shilajit is a pale-brown to blackish-brown organic mineral substance available from Himalayan rocks. We demonstrated that in type I obese humans, shilajit supplementation significantly upregulated extracellular matrix (ECM)–related genes in the skeletal muscle. Such an effect was highly synergistic with exercise. The present study (clinicaltrials.gov NCT02762032) aimed to evaluate the effects of shilajit supplementation on skin gene expression profile and microperfusion in healthy adult females.
Methods: The study design comprised six total study visits including a baseline visit (V1) and a final 14-week visit (V6) following oral shilajit supplementation (125 or 250 mg bid). A skin biopsy of the left inner upper arm of each subject was collected at visit 2 and visit 6 for gene expression profiling using Affymetrix Clariom™ D Assay. Skin perfusion was determined by MATLAB processing of dermascopic images. Transcriptome data were normalized and subjected to statistical analysis. The differentially regulated genes were subjected to Ingenuity Pathway Analysis (IPA®). The expression of the differentially regulated genes identified by IPA® were verified using real-time polymerase chain reaction (RT-PCR).
Results: Supplementation with shilajit for 14 weeks was not associated with any reported adverse effect within this period. At a higher dose (250 mg bid), shilajit improved skin perfusion when compared to baseline or the placebo. Pathway analysis identified shilajit-inducible genes relevant to endothelial cell migration, growth of blood vessels, and ECM which were validated by quantitative real-time polymerase chain reaction (RT-PCR) analysis.
Conclusions: This work provides maiden evidence demonstrating that oral shilajit supplementation in adult healthy women induced genes relevant to endothelial cell migration and growth of blood vessels. Shilajit supplementation improved skin microperfusion.
... 23,24 Although human trials are lacking, restoration of circulation to the heel was evidenced with topical arginine treatments for patients with diabetes. 25,26 Oral and injectable supplementation of arginine is an area of continued research interest. ...
... 10 For the patient with an unstageable ulcer, there is an increased likelihood that a stage III or stage IV ulcer is being masked by eschar in the wound bed. 10 Arginine has shown preliminary benefits for the patient with diabetes and a heel ulcer through its role in NO synthesis, which promotes vasodilation and wound healing. [24][25][26]29,33 Although research is conflicting on ONS in this population, 33 the patient was provided a carbohydrate-controlled formula that contained supplemental arginine to meet nutrient goals. The supplement did not contain additional glutamine or β-hydroxyβ-methylbutyrate, which could have been utilized to assist in wound healing. ...
... This may be contraindicated for the patient with adequate and varied nutrient intake that likely meets micronutrient needs. 32 Although some topical treatments such as arginine, [23][24][25][26]33 erythropoietin, [35][36][37][38] and olive oil 39,40 have shown promise, there is not enough evidence to yet support routine use of these treatments for patients with wounds. ...
Diabetes can increase a patient's risk for the development of a pressure ulcer. Specifically, diabetic heel ulcers require an interprofessional approach that includes nutritional care provided by a dietitian. Assessment of malnutrition risk, along with a review of macro- and micronutrient deficiencies, assists the dietitian in determining the most appropriate intervention for a diabetic patient with a heel ulcer. Standardized nutrition recommendations exist for the management of pressure ulcers; however, a need exists for further evidence to support these guidelines. This case report presents a patient with a diabetic heel ulcer and explores evidence-based nutrition management.
... It was shown that the silicate inositol complex has physicochemical properties that increase the rate of absorption of arginine across the intestinal tract and significantly raise the concentration of available arginine in the circulation, compared with conventional preparations such as arginine hydrochloride [1,3]. It has been also reported in the published literature that arginine has been successfully used as an active ingredient in wound healing cream preparations [9]. Since the ASI complex has been shown to be a superior form of arginine, it is hypothesized that the arginine in the cream provides enhanced blood flow and serves as an active nutrient as opposed to the complex being a protective cover [9]. ...
... It has been also reported in the published literature that arginine has been successfully used as an active ingredient in wound healing cream preparations [9]. Since the ASI complex has been shown to be a superior form of arginine, it is hypothesized that the arginine in the cream provides enhanced blood flow and serves as an active nutrient as opposed to the complex being a protective cover [9]. However, no prior studies have been conducted to determine which active components are released from the ointment. ...
Arginine silicate inositol (ASI) complex is a composition of arginine, silicon, and inositol that has been shown to have beneficial effects on vascular health. This study reports the effects of an ASI ointment on wound healing in rats. A full-thickness excision wound was created by using a disposable 5 mm diameter skin punch biopsy tool. In this placebo-controlled study, the treatment group’s wound areas were covered by 4 or 10 % ASI ointments twice a day for 5, 10, or 15 days. The rats were sacrificed either 5, 10, or 15 days after the wounds were created, and biopsy samples were taken for biochemical and histopathological analysis. Granulation tissue appeared significantly faster in the ASI-treated groups than in the control groups (P < 0.05). The mean unhealed wound area was significantly smaller, and the mean percentage of total wound healing was significantly higher in ASI-treated wounds than in the control wounds. Hydroxyproline, collagen, and matrix metalloproteinases were measured in the granulated tissue and found to be affected. Inducible nitric oxide synthase (iNOS), endothelial nitric oxide synthase (eNOS), collagen, matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), epidermal growth factor (EGF), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and various cytokines (TNF-α and IL-1β) measured in this study showed a significant fall in expression level in ASI-treated wounds. The results suggest that topical application of ASI ointment (especially 4 % concentration) has beneficial effects on the healing response of an excisional wound.
... Одним из таких является аргинин -аминокислота, которая играет важную роль в метаболических процессах организма человека [10]. Доказано положительное влияние аргинина на гипертонию [11,12] репродуктивную физиологию [13], мышечную силу и массу тела [14], почечную и иммунную функции [15,16]. ...
Systemic or oral administration of arginine improves the function of cardiovascular system and reduces myocardial ischemia in patients with coronary heart disease, lowers blood pressure in hypertensive patients and in patients with normal or poor kidney function. Although the concentration of arginine in the plasma do not vary with hypercholesterolemia, oral or intravenous administration of arginine can significantly reduce endothelial dysfunction in patients with hypercholesterolemia and in smokers. The main action Arginina due to its role as a precursor of nitric oxide synthesis. L-arginine affects the pathophysiological mechanisms that exist method, the progression of atherosclerosis. L-arginine can be considered as an integrative part of a multimodal approach to combating acute cerebrovascular pathology, development-it excitotoxicity and ischemic brain injury. Neuroprotective properties of the L-arginine suggests that they may have potential as neuroprotective therapy in stroke patients.
... [8] Arginine (ARG) (2-amino-5-guanidinopentanoic acid) is a conditional essential or semi-essential amino acid which plays an important role in several metabolic pathways in the human. [9] It has been reported to have a positive impact on hypertension, [10,11] reproductive physiology [12] muscle strength and mass, [13] renal and immune function, [14,15] and the release of growth hormone. [16] The role of ARG in solubility enhancement of proteins has been expansively reported by several researchers. ...
Categorized as a Biopharmaceutics Classification System Class II drugs, atorvastatin (ATV) exhibits low aqueous solubility and bioavailability thus presenting an obstacle and great challenge to formulation researchers. Numerous studies are available in regard to the solubility enhancement of ATV, but very few actually describe this phenomenon in terms of thermodynamics and the solute-solvent interaction. Arginine (ARG) is an amino acid that has been reported to enhance the solubility of the highly insoluble wheat protein gluten through hydrogen bonding and π electron-cation interaction. To our knowledge, ARG has never been investigated as a solubility enhancement agent of aqueous insoluble drugs. Thus, this study aimed to elucidate the solute-solvent and solute-cosolute interactions and derive thermodynamic parameters that bolstered the solubility of ATV in the presence of ARG. We examined the electrolytic conductance and densities of ATV-ARG binary system covering the temperature ranging from 298.15 K to 313.15 K. Conductometric and volumetric parameters such as limiting molar conductance, association constants, limiting partial molar volumes, and expansibility values were calculated. Additionally, thermodynamic parameters (ΔG(0), ΔH(0), ΔS(0), and Es) involved in the association process of the solute in the aqueous solution of ARG were also determined.
... 49 A pilot study using L-arginine containing cream to rub the feet of diabetic patients twice daily, for two weeks, showed an improvement of both the temperature and blood flow of the feet. 50 On the other hand, when tried in diabetic patients with peripheral neuropathy (neuropathy disability score > 3), L-arginine (3 g) administered three times daily for 3 months did not show any improvement in the neuropathy disability score. However, there was a larger deterioration in the placebo group compared to the L-arginine group that did not reach statistical significance. ...
Diabetic neuropathy (DN) is a common complication of diabetes mellitus that is hardly reversible at the late stages. Since treatment of neuropathic pain is predominantly symptomatic, a prophylactic measure would be useful. Both ibuprofen and L-arginine exert antiallodynic effects on chronic constriction injury (CCI)-induced cold allodynia. Furthermore, ibuprofen is effective in CCI-induced mechanical allodynia. The aim of the study was to assess the antiallodynic effect of prophylactic ibuprofen and L-arginine in streptozotocin-induced DN in rats and to further investigate the role of spinal miR-155 and NO in this effect. Tactile allodynia was assessed weekly by von Frey filaments. Oral daily administration of ibuprofen, L-arginine and their combination, for 4 weeks starting one week after streptozotocin injection (i.e. before the development of tactile allodynia), resulted in a significant decrease of tactile allodynia compared to the control diabetic group. This was evident in the fifth week of the experiment. The three treatments prevented the decrease in muscle fiber diameter and epidermal thickness, seen in the control diabetic group. Furthermore, ibuprofen. L-arginine and their combination prevented the increase in the spinal NO level and miRNA-155, seen in the control diabetic group. In conclusion, both ibuprofen and L-arginine delayed the development of behavioural and histological changes of DN, with concomitant suppression of spinal miR- 155 and NO level. L-arginine, being tolerable may be useful prophylactically in diabetic patients.
... NO mediates endothelium-dependent vasorelaxation by its action on guanylate cyclase in Vascular Smooth Muscle Cells (VSMC), initiating a cascade that leads to vasorelaxation (Vega-Lopez et al., 2004). Supporting to Science Publications AJPT these findings, supplying of L-arginine transdermally improved vascular function of the feet in patients with diabetes as indicated by flow and temperature (Fossel, 2004). Indeed, antioxidants-rich nutritional supplements could result in favorable effects on the life quality of diabetic patients and patients suffering from other chronic conditions. ...
It was shown that hyperglycemia in diabetic patients is the main factor of diabetic peripheral
neuropathies. Various pathways related to oxidative stress, vascular defect and defective endothelium
dependent relaxation have been implicated in the development of diabetic peripheral neuropathy. A
substantial number of studies have shown that antioxidant treatment are promising therapeutics that can
prevent or correct reduced motor nerve conduction in diabetic rats by acting on these mechanisms. This
study was designed to investigate the possible role of insulin treatment along with or without vitamin E
or L-arginine on diabetic neuropathy. This goal was accessed by examining nerve conduction, parameters
of oxidative stress and lipid peroxidation as well as the expression level of endothelial nitric oxide
synthase in the sciatic nerve of control and streptozotocine-induced diabetic rats. Data showed that
diabetic rats showed increased levels of serum glucose (382.5%) and sciatic nerve lipid peroxidation
Marker (MDA, 261.6%) with a concomitant decrease in the expression of sciatic nerve eNOS mRNA as
compared to control rats. The nerve conduction studies of the sciatic nerves of these rats showed decrease
conduction as evident by delayed NCV (63.6%) and low Amplitude of Muscle Contraction (AMC,
36.4%). Solitary insulin treatment (but not vitamin-E or L-arginine) corrected serum glucose to control
values and corrected nerve conduction parameters in the sciatic nerve. However, treating diabetic rats
with different doses of vitamin E (300 mg kg-1 and 600 mg kg-1) significantly reduced oxidative stress by
decreasing MDA and increasing GPx activity, corrected NCV by reducing the latency and improving
AMC and increased eNOS mRNA expression in sciatic nerve with a more profound effect to seen with
the high dose (600 mg kg-1). However, the maximum potent ameliorating effect of the vitamin E on these
parameters was seen when administered in combination with insulin. On the other hand, L-arginine
treatment alone or in combination with insulin had no effect on the oxidative stress markers nor eNOS
expression but significantly and maximally improved NCV through reducing the conduction latency and
increasing AMC. This study supported the notion that diabetic peripheral neuropathy is a multifactorial
complication, caused by hyperglycemia, oxidative stress and vascular impairment. It is concluded that
conjugate treatment with vitamin-E, especially in higher doses, with insulin could be of great value.
Moreover correction of impaired nerve blood flow by drugs that induce nitric oxide has proved to be
efficient in the protection against and correction of experimental diabetic peripheral neuropathy.
... l-arginine is used in many medical problems such as congestive heart failure, angina pectoris, erectile dysfunction, preventing inflammation of the digestive tract in premature infants, intermittent claudication, pre-eclampsia, improving athletic performance, sickle cell disease, and improving healing of diabetic foot ulcers [15,16]. ...
... Other than the benefits in the above stated conditions, l-arginine has been demonstrated to improve peripheral circulation [78], renal function [79], and immune function [80]. It also possesses antistress and adaptogenic capabilities [81]. ...
l-Arginine is one of the most metabolically versatile amino acids. In addition to its role in the synthesis of nitric oxide, l-arginine serves as a precursor for the synthesis of polyamines, proline, glutamate, creatine, agmatine and urea. Several human and experimental animal studies have indicated that exogenous l-arginine intake has multiple beneficial pharmacological effects when taken in doses larger than normal dietary consumption. Such effects include reduction in the risk of vascular and heart diseases, reduction in erectile dysfunction, improvement in immune response and inhibition of gastric hyperacidity. This review summarises several positive studies and personal experiences of l-arginine. The demonstrated anti-aging benefits of l-arginine show greater potential than any pharmaceutical or nutraceutical agent ever previously discovered.
Nitric oxide is a small molecule gas that is primarily produced by epithelial cells throughout the body (e.g., the inner lining of blood vessels, the blood-brain barrier, and barriers in the gut and around reproductive structures). Nitric oxide is known as the universal messenger, made in ubiquitous quantities to signal both defense mechanisms blocking harmful substances from entering the body and the mechanisms that permit helpful substances to enter the body. As such it is involved in many processes, for example, detoxification and the urea cycle, tissue regeneration, blood flow, prevention of atherosclerosis, and regulation of inflammation and oxidation. A large reservoir of nitric oxide is very important to health and wellness, but not too large. As with everything else in life, there still needs to be a proper balance. However, with the typical Western lifestyle, most do not have nearly as large a reservoir as is needed for wellness. Therefore, supplements are strongly recommended.
