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Effect of incubation time on the biomass production of aquaculture probiotic strains in basal media supplemented with fermented rice extract
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Traditional commercial media are costly and not ideal for large-scale probiotic production in aquaculture. Choosing the right carbon source for probiotic growth and understanding how biomass production works can offer valuable insights for potential use in aquaculture. In this study, we tested 12 starchy extracts as potential cost-effective alterna...
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In shrimp farming, the development of alternative production systems, such as fermented products, can help reduce the high dependence on artificial diets and ensure more sustainable growth. The “synbiotic system” typically uses low stocking densities in traditional earth ponds. To increase profitability, the tendency is to increase stocking densiti...
Citations
... In their growth, probiotics require an energy source, one of which comes from carbon (Sasikumar et al., 2024). Carbon sources include macronutrients essential for microorganisms to stay alive (Wicaksono et al., 2017). ...
The study investigated the optimal dose of molasses for the cultivation of commercial probiotics containing Bacillus amyloliquefaciens and Bacillus subtilis. A two-month laboratory experiment was conducted at the Faculty of Fisheries and Marine Sciences, Jenderal Soedirman University, using a Completely Randomized Design (CRD) with four treatments: K (0 ppm), A (5 ppm), B (10 ppm), and C (15 ppm), each with three replications. The study measured optical density (OD) and bacterial growth over 48 hours. The results showed that Treatment C (15 ppm) produced the highest OD value (0.014), indicating substantial bacterial growth. However, it exhibited the slowest growth phase. In contrast, Treatment A (5 ppm) demonstrated the fastest growth phase and achieved the highest bacterial density throughout the study. This suggests that 5 ppm of molasses is ideal for rapid bacterial proliferation. Treatment K (0 ppm) resulted in the lowest OD (0.0042) and led to bacterial death by the end of the experiment, highlighting the necessity of molasses for bacterial survival and growth. In conclusion, a 5 ppm molasses dose (Treatment A) proved to be the most effective for promoting optimal bacterial growth and density, while higher doses resulted in slower growth, and no molasses inhibited bacterial survival.
