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Effect of increasing coagulant concentrations on: A N1 copies/g extracted mass, B N2 copies/g extracted mass, C PMMoV copies/g of extracted mass, D N1 copies/L of total sample volume, E N2 copies/L of total sample volume, F PMMoV copies/L total sample volume, G N1 copies/copies PMMoV, and H N2 copies/copies PMMoV. Note: The concentrations of coagulant, denoted as C1, C2, C3, C4, and C5, corresponding to 0, 5, 15, 30, and 60 mg/L as Fe³⁺, respectively

Effect of increasing coagulant concentrations on: A N1 copies/g extracted mass, B N2 copies/g extracted mass, C PMMoV copies/g of extracted mass, D N1 copies/L of total sample volume, E N2 copies/L of total sample volume, F PMMoV copies/L total sample volume, G N1 copies/copies PMMoV, and H N2 copies/copies PMMoV. Note: The concentrations of coagulant, denoted as C1, C2, C3, C4, and C5, corresponding to 0, 5, 15, 30, and 60 mg/L as Fe³⁺, respectively

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Wastewater surveillance (WWS) of SARS-CoV-2 has become a crucial tool for monitoring COVID-19 cases and outbreaks. Previous studies have indicated that SARS-CoV-2 RNA measurement from testing solid-rich primary sludge yields better sensitivity compared to testing wastewater influent. Furthermore, measurement of pepper mild mottle virus (PMMoV) sign...