Figure - available via license: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Content may be subject to copyright.
Effect of different concentrations of BA on shoot induction from nodal explants of R. cordifolia after 30 days of inoculation
Source publication
Plants are cherished as the chief resource for healthcare products attributed to synthesizing diverse array of bio-molecules through various metabolic alterations. Over exploitation of such resources can intimidate its existence. Developing medicinal plants into crops and the use of micropropagation as technique to mass produce high-demand biomass...
Context in source publication
Context 1
... concentration of BA exceeding 2.5 µM significantly (p < 0.001) reduced the shoot length, which may be due to the inhibition of adventitious meristem by BA at higher concentration by shifting gene activation [4]. Cultures raised in 2.5 µM BA produced 3.26 shoots per culture; shoots were grown in to 3.14 cm size accordingly with 2.51 shoot forming capacity (Table 1) (Table 2). The comparison of cytokinins showed superior performance of BA for R. cordifolia. ...Citations
... In R. cordifolia, various phytohormones like BAP, Kn, BAP + IAA, and BAP + NAA have been tested for shoot multiplication and rooting, and the maximum shoot number (5.9 and 5.2 per explant) was observed on medium containing 1.0 mg/L BAP and 0.02 mg/L IAA (Radha et al. 2011). Devi Priya and Siril (2022) also reported rapid axillary bud proliferation with 2.5 µM BA and TDZ, yielding 9.37 shoots. However, these rates are lower than the results achieved with mT in this study. ...
This pioneering study focuses on assessing the impact of meta-topolin (mT) hormone on the in vitro shoot proliferation, rooting, and genetic fidelity of Rubia cordifolia L. commonly known as Manjistha or Indian Madder to ultimately refine the micropropagation strategy. A significant positive residual effect of optimum mT concentration on in vitro rooting in R. cordifolia was also demonstrated. Sixteen different growth medium combinations were tested, including the basal MS medium supplemented with various concentrations of mT (ranging from 0.05 to 5.0 mg/L) and other cytokinins (BAP, Kn, 2-iP at 1.0 mg/L concentration) for in vitro shoot proliferation. R. cordifolia demonstrated 100% shoot induction across all the tested medium combinations. The concentration of mT significantly influenced shoot production, with the 2.0 mg/L concentration proving most effective, yielding the highest number of shoots (13.17±0.27) and nodes (28.44±0.71) after 16 wk, which further increased to 26.83±0.35 shoots and 48.73±0.70 nodes after 24 wk. Optimal in vitro rooting was achieved with 2.0 mg/L IBA, producing the maximum number of roots (18.50±0.48) after 8 wk. The residual effect of various cytokinins, including mT, on in vitro rooting was also investigated. Micro-shoots multiplied using 2.0 mg/L mT generated the best results, yielding a significantly higher number of roots (20.22±0.62) than micro-shoots multiplied using half basal MS. During acclimatization, all the rooted plants displayed 100% survival. Genetic integrity assessment using 20 ISSR markers demonstrated no discernible differences in the banding patterns between the mother plant and the in vitro regenerated plantlets. This optimized methodology has the potential for large-scale propagation of uniform-quality disease-free madder planting material to not only reduce the pressure on natural resources but also replenish its natural populations.
