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Effect of RJ on mitotic index and frequency of chromo- somal aberrations in Allium cepa L. root tip meristems.
Source publication
The effect of royal jelly (RJ) on the seed germination, seedling growth (radicle length, radicle number and fresh weight), mitotic activity and chromosomal aberrations of Allium cepa L. germinated under both normal conditions and salt stress were investigated in this work. The radicle length and fresh weight of the seeds germinated in the medium wi...
Contexts in source publication
Context 1
... all values mentioned here are substantially significant. The mitotic index and chromosomal aberra- tion scores obtained from the control and treated seeds are summarized in Table 2. ...
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... is, 0.3 mg L 1 RJ ap- plication showed a perfectly successful result on the mi- totic activity by accelerating cell division. However, the frequency of chromosomal aberrations increased fivefold with this dose of RJ application (Table 2). In this case, we can say that some aberrations may result from this stimulator, and 0.3 mg L 1 RJ application has a genotoxic effect on A. cepa root tip meristems. ...
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... example, in 0.15 M NaCl medium, RJ application significantly increased the fresh weights of the seedlings compared to the control (Table 1). Moreover, it reduced the preventive effect of salt on the seed germination and seedling growth by stimulating mitotic activity of the embryo (Table 2). It could have made an attack against the ABA being a germination inhibitor whose amount probably increases due to the salt existence. ...
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... data indicated that salin- ity according to control decreased the mitotic index by 66% and resulted in a higher number of chromosomal abnormalities. The frequency of aberrations by salinity increased twice as compared to control group (Table 2). However, 0.3 mg L 1 RJ application exhibited a perfect performance by alleviating the inhibitive effect of salin- ity on the mitotic index. ...
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... contrast, RJ application could not be successful in decreasing the detrimental ef- fect of salinity on the chromosomal aberrations. The fre- quency of aberrations in root tip cells of the seeds ger- minated in RJ application together with salinity (group IV) was more than approximately fivefold higher than in the control (Table 2). However, RJ+NaCl application (group IV) decreased the detrimental effect of salinity on aberrations by approximately 8% compared to the application of RJ alone (group III). ...
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... the result proves that RJ used at convenient concentrations might help to alleviate the genotoxic effects of salt stress. The most striking aberrations observed in Group IV (0.3 mg L 1 RJ +0.15 M NaCl) were also bridge formation and fault polarization, whereas the minimal common aberrations were micronuclei and disrupted equatorial plate (Table 2). ...
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Citations
... Significant increase in growth parameters and an alternation in the expression of stress associated genes were recorded in snap bean, Pisum sativum, Hordeum vulgare and flax Plants treated with FA [27][28][29]. Royal jelly which is released from glands on the worker bees' heads as queens' diet, has high nutritional and antioxidant properties [30] and play the main role in differential variations between queen and workers in bees (Apis millifera). These differential variations are accomplished by epigenetic regulation as DNA methylation of different diet [22]. ...
... Increasing CA% under RJ treatment was reported as a result of its stimulation effect and it was directly proportional to the increase in MI% ( Table 3). Using of convenient doses of RJ may assist in reducing the hazardous effects of salinity stress [30]. Considering properties of RJ components which provide stabilization of cell membranes and raise antioxidant activities could elucidate the increase in cell division, growth, nucleic acid and protein synthesis and support its recovery role and reduction of CAs on stressed plants [49,67]. ...
... Royal jelly was able to reduce the inhibitory effects of salinity as it was associated with a significant decrease in MT1 and MT2 expression at both studied varieties comparing with untreated stressed plants. Using of RJ at convenient doses may assist in decreasing the harmful effects of salinity stress [30]. It is clear that RJ has a major epigenetic impact in the differentiation of the larvae into workers and queens [81]. ...
DNA methylation is one of induced changes under salinity stress causing reduction in the expression of several crucial genes required for normal plant's operation. Potential use of royal jelly (RJ), folic acid (FA) and 5-azacitidine (5-AZA) on two Egyptian faba bean varieties (Sakha-3 and Giza-716) grown under saline conditions was investigated. Salinity stress affects negatively on seeds germination (G %), mitotic index, membrane stability and induced a significant increase in chromosomal abnormalities (CAs). DNA methyltransferases genes (MT1 and MT2) were highly up-regulated (∼23 and 8 folds for MT1 and MT2 in shoots of Giza-716 stressed plants). On the other hand, down regulation of other studied stress related genes: superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), heat shock protein (HSP-17.9) and proline-rich protein (GPRP) were detected in stressed plants of both studied varieties. Treating plants with RJ and FA increase G%, chlorophyll content, improves membrane properties and reduces CAs compared to non-treated stressed plants. Exogenous application of 5-AZA, RJ and FA on salinity stressed plants was associated with a significant reduction in the transcription of MT1 and MT2 which was associated with significant up regulation in the expression of Cu/Zn-SOD, CAT, GR, GPRP and HSP-17.9 encoding genes. The Lowest expression of MT1 and MT2 were induced with 5-AZA treatment in both studied varieties. Exogenous application of the FA, RJ and 5-AZA modified the methylation state of stressed plants by regulation the expression of DNA methyltransferases, subsequently, modulated the expression of studied genes and could be proposed as a promising treatment to ameliorate hazardous effects of salt stress on different plants.
... One of the most important way to reduce the harmful effects of abiotic stresses on plant cultivation is to produce plant varieties resistant to these stress factors. Therefore, various plant hormones, growth regulators and vitamins, which have protective function have been used in numerous studies to diminish or minimise harmful effects of abiotic stresses (Çavuşoğlu et al. 2017, Noman et al. 2018, Özmen and Tabur 2020. However, the correlation between these protectors used and tolerance of abiotic stresses is quite complex and not always apparent in plants. ...
Impacts of the exogenously applied polyamines (PAs) on some physiological features and proline content in drought-resistant barley cultivars (Hordeum vulgare cv. Bülbül-89) and drought-susceptible (Hordeum vulgare cv. Burakbey) were comparatively examined under PEG-6000-simulated drought stress conditions. It was found that drought stress application had adverse impact on all physiological parameters at least 40% compared to that of the control group. It also dramatically increase the proline content of the cultivars. However, exogenously applied PAs (especially Spm on both barley cultivars) under drought stress conditions showed positive effect on all physiological parameters studied. The proline content increased in the presence of all PAs (especially Spd) in drought-susceptible Burakbey cultivar. If in Bülbül-89, polyamine that increases the proline content was Spd only. Among polyamines Cad was found as the polyamine that provided generally the least successful on all parameters.
... RJ comprises water (50-70 %), proteins (9-18 %), carbohydrates (7-18 %), fatty acids and lipids (3-8 %), mineral elements (1.5 %) [27][28][29][30] . RJ application reduced the detrimental effect of salt on studied traits in dramatically varying degrees while studying the influence of RJ (concentration of 0.3 mg L -1 from 60 capsules containing 1500 mg of freeze-dried RJ) on the seed germination, seedling growth and mitotic activity of Allium cepa L. germinated under both normal and salt stress conditions 31 . The response of vegetative growth and flowering attributes, chemical constituents, and oil composition of calendula plants to 4 levels of propolis aqueous extract foliar spray (0, 5, 10, 15 and 20 g L -1 ) were investigated 32 . ...
Two consecutive field trials (2019 and 2020) were conducted to investigate the effect of foliar spraying of algae and royal jelly extracts on growth, essential oil content and composition of lemon verbena (Aloysia Citriodora) plant. Three concentrations of algae extract (0, 0.5 and 1 cm³L⁻¹) and three concentrations of royal jelly (0, 1 and 2 gL⁻¹) were used. All algae and royal jelly treatments positively impacted growth characters of lemon verbena (Aloysia Citriodora) plant viz, plant height, fresh and dry weights of herb and essential oil contents in both harvests during the two seasons. Plants sprayed with 0.5 cm³L⁻¹ algae and 1 gL⁻¹ royal jelly extracts produced the highest herb fresh and dry weights and essential oil contents. D-Limonene percent in the essential oil varied from 21.4 to the maximum of 25.9 % when plants were sprayed with 1 cm³L⁻¹ of algae + 1 gL⁻¹ of RJ. Geranial and neral maximally enhanced by 1 gL⁻¹ without algae spraying which recorded 12.5 and 9.8 % respectively.
... Moreover, it contains at least 17 amino acids as well as, the important minerals, such as potassium, magnesium, calcium, iron, phosphorus, sulfur, manganese and silicon (Nation and Robinson, 1971;Lakin, 1993, El-Shaikh, 2010Pasupuleti et al., 2017). There are several studies strongly suggest the important role of RJ in improving growth, yield and quality of horticultural crops and protect plants from various stresses (El-Maziny and Hassan, 1990;El-Shaikh, 2010;Wassel et al., 2015;Dalia and El-Aref, 2016;Çavuşoğlu, et al., 2017). So, the aim of this study was to evaluate the potential effects of foliar spray with two biostimulants (chitosan and royal jelly) on the growth, yield and quality of two garlic cultivars under El-Minia governorate, Egypt conditions. ...
... The positive effect of royal jelly on total yield (ton/feddan) may be attributed to its higher content from nutrients, amino acids, lipids, fructose, glucose, sucrose, vitamins and fats (El-Shaikh, 2010;Ibrahim et al., 2015). Our results are in accordance with previously reported results of (El-Shaikh, 2010; Dalia and El-Aref, 2016;Çavuşoğlu, et al., 2017). ...
This study was conducted to evaluate the potential effect of foliar spray with different doses of two bio-stimulants Chitosan (CS) and Royal Jelly (RJ) on growth and yield and quality of two garlic cultivars (Egaseed1 and Balady i.e( Egyptian) at two winter seasons of 2017/2018 and 2018/2019.Generally, the foliar spray of all tested bio-stimulates treatments considerably improves almost all plant growth traits of garlic cultivars which reflected on plants yield and quality. Data showed that spraying Ega1 plants with different doses of CS and RJ considerably increased total yield (TY) in both seasons as compared with control (13.39 and 14.64 tons). The highest values of TY were found in Ega1 plants treated with 0.1g/l RJ (15.29 and 15.80 tons) and 0.3g/l RJ (15.08 and 16.61 tons) at both seasons respectively. On the other side, there was clear increase in TY of Egy plants treated with different CS and RJ doses at both seasons as compared with control (7.57 and 8.69 tons, respectively). The highest values of TY were found in Egy. plants treated with 0.2g/L RJ (9.23 and 8.53 tons) at both seasons respectively as compared with all other treatments. As compared to control, foliar spray with CS and RJ reduced weight loss % and deterioration of bulbs during storage. In general, foliar application of chitosan (1000 and 1500 ppm) and Royal Jelly (0.2 and 0.3 g.L-1) could be recommended to improve productivity, quality and storability of the two garlic cultivars grown under Minia Governorate conditions
In this study, the protective role of royal jelly (RJ) against the potential toxic effects of sodium benzoate was investigated in Allium cepa L. test material with physiological, genetic, and biochemical parameters. Physiological changes were evaluated by determining weight gain, rooting percentage, root length, and relative injury rate. The genetic evaluations were carried out with chromosomal abnormalities (CAs), micronucleus (MN), tail DNA formation, and mitotic index (MI) ratio parameters. The biochemical evaluations were carried out by determining lipid peroxidation and antioxidant enzyme activities by determining levels of malondialdehyde (MDA), glutathione reductase (GR), superoxide dismutase (SOD), and catalase (CAT). Further, the interaction of sodium benzoate with antioxidant enzymes was evaluated with molecular docking analysis. The antimutagenic effect of RJ was evaluated as the inhibition of chromosomal abnormalities (CAs) and tail DNA formations. A total of six groups were formed in the study. A. cepa L. bulbs in the control group were treated with tap water; the bulbs in the administration groups were treated with sodium benzoate (100 mg/L), RJ (25 mg/L and 50 mg/L doses), and sodium benzoate–RJ combinations with these doses for 72 h. As a result, it was determined that sodium benzoate application caused inhibition of physiological parameters and MI; induced MN, CAs, and DNA damage; and also caused oxidative stress. Depending on the concentration of RJ application, it reduced sodium benzoate toxicity by showing therapeutic effects in all these parameters. Also, the interaction of sodium benzoate with antioxidant enzyme residues was determined by molecular docking analysis. As a result, it has been understood that abandoning the use of sodium benzoate will be beneficial for the environment and human health and concluded that the use of RJ in the daily diet will be effective in reducing the impact of exposed toxic ingredients.
