Effect of PEI–pSDF-1α transfection on HUVECs and its impact on ADSCs. A PEI–pSDF-1α transfected HUVECs exhibited significantly enhanced survivability response compared to those transfected nontherapeutic PEI–pLuc polyplex. B Transfection with PEI–pSDF-1α induced transient production of the target protein over a period of 2 weeks. C Phase-contrast microscopy images of morphological changes in HUVECs and its coculture with ADSCs at day 14. i Untransfected HUVECs maintained its cobblestone-like morphology. ii Transfected HUVECs appeared more polarized. iii Addition of ADSCs to untransfected HUVECs resulted in the formation of interconnected cellular network within the monolayer assembly. iv Elongated, pseudo three-dimensional dense cellular clusters were formed in transfected coculture group. Data are plotted as mean ± standard deviation (n = 6)

Effect of PEI–pSDF-1α transfection on HUVECs and its impact on ADSCs. A PEI–pSDF-1α transfected HUVECs exhibited significantly enhanced survivability response compared to those transfected nontherapeutic PEI–pLuc polyplex. B Transfection with PEI–pSDF-1α induced transient production of the target protein over a period of 2 weeks. C Phase-contrast microscopy images of morphological changes in HUVECs and its coculture with ADSCs at day 14. i Untransfected HUVECs maintained its cobblestone-like morphology. ii Transfected HUVECs appeared more polarized. iii Addition of ADSCs to untransfected HUVECs resulted in the formation of interconnected cellular network within the monolayer assembly. iv Elongated, pseudo three-dimensional dense cellular clusters were formed in transfected coculture group. Data are plotted as mean ± standard deviation (n = 6)

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... The lymphangiogenic function increases with infiltrating macrophages that produce (VEGF-C) [35]. In addition to lymphangiogenesis, VEGF-C may regulate physiological angiogenesis [35,36]. Studies have also shown that SIM can suppress the growth of bacterial pathogens [37,38]. ...
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