Figure 2 - available via license: Creative Commons Attribution 4.0 International
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Effect of MTX or MTX+Anti-Notch2 antibody (ab) treatment on bone marrow sinusoid dilation in rats (histological analyses). (A) Representative tibial sections with H&E staining from different treatment groups, showing treatment effects on bone marrow sinusoids (indicated by yellow dash lines). Scale bar is 100 µm. (B) BM sinusoidal diameters at different treatment groups. (C) Treatment effects on sinusoidal area per bone marrow area. *** p < 0.001 and **** p < 0.0001 compared to control group; ## p < 0.01 and #### p < 0.0001 compared with MTX+Control IgG-treated group. All the measurements are expressed as mean ± SEM.
Source publication
Intensive cancer chemotherapy is well known to cause bone vasculature disfunction and damage, but the mechanism is poorly understood and there is a lack of treatment. Using a rat model of methotrexate (MTX) chemotherapy (five once-daily dosses at 0.75 mg/kg), this study investigated the roles of the Notch2 signalling pathway in MTX chemotherapy-ind...
Contexts in source publication
Context 1
... shaped mono-layer thin-walled micro-vessels in the lower secondary spongiosa of metaphysis bone have been considered as sinusoids [16,17]. Tibial histological image analyses illustrated a very significant (p < 0.0001) sinusoidal dilation in the lower secondary spongiosa at day 9 following the first MTX treatment in MTX+Control IgG group compared to the control (Figure 2A,B). However, anti-Notch2 treatment remarkably attenuated MTX-induced sinusoidal dilation, when compared to MTX+Control IgG group (p < 0.0001) ( Figure 2B). ...
Context 2
... histological image analyses illustrated a very significant (p < 0.0001) sinusoidal dilation in the lower secondary spongiosa at day 9 following the first MTX treatment in MTX+Control IgG group compared to the control (Figure 2A,B). However, anti-Notch2 treatment remarkably attenuated MTX-induced sinusoidal dilation, when compared to MTX+Control IgG group (p < 0.0001) ( Figure 2B). Furthermore, the same trends of treatment-induced changes were observed in sinusoidal area per marrow area. ...
Context 3
... the same trends of treatment-induced changes were observed in sinusoidal area per marrow area. There was remarkable increase in the size (area) of vasculature per marrow area in MTX+Control IgG group (p < 0.001) compared to control, which was prevented by the anti-Notch2 antibody supplementation (p < 0.01) when compared to MTX+Control IgG group ( Figure 2C). ...
