Figure 3 - uploaded by Sachin Jain
Content may be subject to copyright.
Effect of Boerhaavia diffusa root and Silybum marianum seeds extracts on alkaline phosphatase of high fructose diet induced hepatotoxicity in mice. Values are in Mean ± standard error of mean (n = 6) ANOVA followed by Tukey's Test. ***P < 0.001 vs. control group # P < 0.05, ## P < 0.01, ### P < 0.001 vs. HFD model control group
Source publication
Aim: The present study was undertaken with a view to validate the traditional use of Boerhaavia diffusa (BEE) root and Silybum marianum (SME) seeds in combination as a hepatoprotective agent against non-alcoholic fatty liver disease. Materials and Methods: The alcoholic extracts of BEE roots (150 mg/kg, p.o.) and SME (150 mg/kg, p.o.) seeds were ad...
Similar publications
A number of recent molecular studies have highlighted divergences between currently recognized genera of lizards within the four closely associated lizard families Xenosauridae, Anniellidae, Diploglossidae and Anguidae, which share the same clade as Heloderma, reviewed by Hoser (2013a) and more distantly Varanidae reviewed by Hoser (2013b)
Combinin...
Bruggmannia >chapadensis sp. nov. is described and illustrated based on characters of the larva, pupa and adults of both sexes, from specimens collected in Cerrado areas of Parque Nacional da Chapada dos Guimarães, state of Mato Grosso, Brazil. The new species is characterized by tarsal claws bent at distal 1/3; well-developed empodia, not reaching...
Allopatry among closely related congeners is common, but is sometimes an artifact attributable to geographic sampling bias. In newly recognized zones of sympatry, congeneric species pairs may show character displacement or, alternatively, may show reduced differentiation suggesting ongoing gene flow. The arboreal alligator lizards Abronia graminea...
We describe a striking new lizard of the genus Abronia from the Sierra de Los Cuchumatanes of Guatemala. Adults of the new Guatemalan species have a unique body pattern of white or yellow transverse markings on a black background and a suite of morphological characters that differentiate them from all congeners. It is the only species of Abronia kn...
Citations
... As, steatosis is the start of a domino effect prior to NASH, a decrease in its induction might halt further progression of the liver disease. The plant components present in Livogrit namely Boerhavia diffusa, Phyllanthus niruri and Solanum nigrum (ratio of 2:1:1) in their individual capacity reportedly ameliorate fatty liver and prevent its further progression to fibrosis [57][58][59]. Previously, we have shown that Livogrit decreases lipid accumulation in non/alcoholic fatty liver disease [15,16]. In this study also, Livogrit was able to markedly reduce lipid accumulation in HepG2 spheroid model. ...
The prevalence of nonalcoholic steatohepatitis (NASH), characterized by fatty liver, oxidative injury, and inflammation, has considerably increased in the recent years. Due to the complexity of NASH pathogenesis, compounds which can target different mechanisms and stages of NASH development are required. A robust screening model with translational capability is also required to develop therapies targeting NASH. In this study, we used HepG2 spheroids and rat primary hepatocytes to evaluate the potency of Livogrit, a tri-herbal Ayurvedic prescription medicine, as a hepatoprotective agent. NASH was developed in the cells via methionine and cystine-deficient cell culture media. Livogrit at concentration of 30 µg/mL was able to prevent NASH development by decreasing lipid accumulation, ROS production, AST release, NFκB activation and increasing lipolysis, GSH (reduced glutathione), and mitochondrial membrane potential. This study suggests that Livogrit might reduce the lipotoxicity-mediated ROS generation and subsequent production of inflammatory mediators as evident from the increased gene expression of FXR, FGF21, CHOP, CXCL5, and their normalization due to Livogrit treatment. Taken together, Livogrit showed the potential as a multimodal therapeutic formulation capable of attenuating the development of NASH. Our study highlights the potential of Livogrit as a hepatoprotective agent with translational possibilities.
... The liver plays a pivotal role in the catabolism of nutrients as well as drugs 6 . Fructose intake stimulates de novo lipogenesis which induces lipotoxicity at insulinsensitive tissues. ...
... Moreover, the treatment of fatty liver in diabetes and obesity is with ursodiol, gemfibrozil, clofibrate, vitamin E, and insulin sensitizers are recommended, but still, complications exist. Hence the management of IR in the initial stages may halt the progression of hepatic steatosis that may be achieved with medicinal plants 6,8 . ...
... These transaminases are usually present in the cytoplasm of the liver and leaked into the bloodstream in conformity of hepatocellular damage. LDH is a cytoplasmic enzyme widely distributed in all the cells and serves as a marker of both hepatic steatosis and cardiac damage 6,39 . As compared to HFrC, liver weights, volumes, and HI and activity of transaminases and LDH in all the treatment groups were restored to near normalcy shows their reversal effects against HFr dietinduced hepatotoxicity. ...
High fructose (HFr) dietary consumption is linked with insulin resistance (IR) and associated complications are documented in both animals and humans. The present study is to evaluate the combined effects of E. Jambolana and C. zeylanicum extracts in IR associated structural and functional hepatic changes in HFr fed rats. Male Wistar rats were randomly grouped into five (n=6). Group 1 received distilled water as normal control (NC), group 2 received HFr diet (60%w/v) as HFr control and group 3 received metformin (MET 500mg/kg), while group 4 received Jamun seed and cinnamon bark aqueous extracts (JSAE+CBAE 500mg/kg of each) and group 5 received Jamun seed and cinnamon bark ethanolic extracts (JSEE+CBEE 500mg/kg of each) respectively. All the rats except NC, fed with HFr diet for 42 consecutive days and respective treatments were administered orally, from day 28 onwards for the next 14 days while maintaining them on HFr diet. Physical and biochemical parameters such as fasting glucose, insulin, triglycerides (TG's), Serum Glutamic Pyruvate Transaminase (SGPT) and Oxaloacetic Transaminase (SGOT), Lactate Dehydrogenase (LDH) and C-reactive protein (CRP) and, Malondialdehyde (MDA) levels were measured. Liver histopathological studies were accessed for structural changes. HFr fed rats significantly (P<0.05) induced hyperglycemia, hypertriglyceridemia, hyperinsulinemia, and with impaired glucose tolerance evidenced by β-cell dysfunction and decreased insulin sensitivity (IS) indicates IR. HFr feeding had elevated hepatic markers, oxidative stress, and inflammation, altogether promotes fatty changes liver in IR rats. JSAE+CBAE and JSEE+CBEE groups significantly (P<0.05) reversed the aforementioned metabolic dysregularties and partially reversed fatty liver changes, and these results were comparable to MET. In conclusion, the combined effects of Jamun and cinnamon extracts exert antihyperglycemic, antitriglyceridemic, antioxidant, anti-inflammatory, and anti-steatosis properties in HFr fed rats. Thus, it offers a promising value in the management of IR associated hepatic changes.
... The leaf extracts have been reported to contain anti-hyperglycemic activities in various animal models [17,18]. The root extract has also been reported to show no α-amylase inhibitory activity [19], but when it was used in combination with seeds of Silybum marianum it showed hepatoprotective abilities on animals with induced non-alcoholic fatty liver disease [20]. The extracts from the leaves and roots of this plant have also been tested on animal models to investigate their anti-hyperglycemic abilities and B. diffusa has also shown some inhibitory effects on carbohydrate absorption in experimental animals [21,22]. ...
The present study investigated the in vitro and ex vivo antioxidant, anti-diabetic and anti-obesogenic potentials of different solvent (ethyl acetate, ethanol and water) extracts from the aerial parts of Boerhaavia diffusa. The ferric reducing antioxidant power (FRAP), DPPH scavenging activity and the ameliorative effects of the extracts on Fe2+-induced oxidative injury was investigated both in vitro and ex vivo. Alpha glucosidase and pancreatic lipase inhibitory potentials of the extracts were examined in vitro, while the effects of the ethanol extract on abdominal glucose intake and muscle glucose uptake were determined in freshly harvested tissues ex vivo. The extracts were subjected to Gas Chromatography-Mass Spectrometry (GC–MS) analysis to identify their possible bioactive components. The ethanol extract showed the most potent FRAP and DPPH radical scavenging activities compared to other extracts. All extracts increased catalase and SOD activities, and GSH levels in oxidative pancreatic injury. Both ethanol and aqueous extracts exhibited remarkable enzyme inhibitory activities, which was significantly higher than ethyl acetate extract and acarbose but was not comparable to orlistat. The ethanol extract portrayed a dose-dependent inhibitory effect on jejunal glucose uptake and enhancement of muscle glucose uptake. 9-(4 methoxyphenyl) xanthene, xanthone and stigmasterol showed strong binding affinities for α-glucosidase
and lipase enzymes tested. Data from this study suggest that aerial parts of B. diffusa (particularly the ethanol extract) may not only exhibit antioxidant potentials but may also mediate anti-lipidemic and anti-hyperglycemic effects via inhibiting fat and carbohydrate digestion as well as abdominal glucose intake and enhancing muscle glucose uptake.
