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Effect of 1 g/L peanut oil on the production of butenyl-spinosyn
(a) Relative production on the 3rd, 5th and 7th days when adding oil at the start of fermentation; (b) The HPLC profiles of butenyl-spinosyn. The detection wavelength was set at 244 nm and the chromatographic peak of butenyl-spinosyn appeared at 7.9 min; (c) Synthesis rate of butenyl-spinosyn; (d) Relative production of butenyl-spinosyn with oil added at different times; (e) The biomass of Sa. pogona; (f) Intracellular malonyl-CoA level comparison; (g) Transcription levels of butenyl-spinosyn synthesis genes.

Effect of 1 g/L peanut oil on the production of butenyl-spinosyn (a) Relative production on the 3rd, 5th and 7th days when adding oil at the start of fermentation; (b) The HPLC profiles of butenyl-spinosyn. The detection wavelength was set at 244 nm and the chromatographic peak of butenyl-spinosyn appeared at 7.9 min; (c) Synthesis rate of butenyl-spinosyn; (d) Relative production of butenyl-spinosyn with oil added at different times; (e) The biomass of Sa. pogona; (f) Intracellular malonyl-CoA level comparison; (g) Transcription levels of butenyl-spinosyn synthesis genes.

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Butenyl-spinosyn discovered from Saccharopolyspora pogona, is a broad-spectrum bioinsecticide. In order to further improve the production, the fermentation medium of a high-production strain Sa. pogona ASAGF30A11 obtained by mutagenesis, was optimized by adding different species and concentrations vegetable oil. In our study, the effect of peanut o...