Dose response of contact inhibition antiviral activity of F18 EC16 nasal formulation. The formulations were diluted from an F18 glycerol stock of EC16 (1%) into serum-free EMEM to a concentration of 0.05, 0.125, 0.25, 0.625, and 1.25 mM prior to incubation with OC43 virus at a 1:9 ratio (virus to formulation) for 30 min, followed by a series of 10 X dilutions and TCID50 assay. The contact inhibition antiviral activity was calculated and expressed as log 10 reduction of the original titer (log 10 9.25/ml), determined in a positive control on the same plate. Results are the mean from three independent experiments (bars show standard deviation).

Contexts in source publication

Context 1

... of virus with dilutions of F18 demonstrated a dosedependent increase in contact inhibition antiviral activity of the formulation, as represented by an increase in the log 10 reduction in viral titer (Figure 3). A plateau in viral titer reduction was evident at higher doses. ...

Context 2

... F18 formulation diluted in EMEM showed potent antiviral activity when virus was directly exposed to it, with a maximum inhibitory effect of log 10 4.21+0.12 (SE) (99.996%) at saturating concentrations in the 1 mM range (Figure 3). Based on a regression analysis and determination of the curve constants, a concentration of 0.225 mM was predicted to give a log 10 3.79 reduction (90% of maximum). ...

Context 3

... this report, a series of initial tests for EC16m was performed with the F18m formulation of EC16m in serum-free EMEM dilutions. After a 30 min incubation with 1.41 mM (0.1%) EC16m the infectivity of the OC43 virus was reduced by 99.9%, similar to that seen with EC16 (99.996%, (Figure 3) at a moderately higher dose. The measured log 10 reduction value was influenced by the titer of the virus used in the tests, with a lower titer resulting in a lower proportionate log 10 reduction. ...