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Distribution of nucleotide changes and the resulted amino acid substitutions of each isolate.

Distribution of nucleotide changes and the resulted amino acid substitutions of each isolate.

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Fluconazole was used to test the susceptibility of Candida albicans isolated from different clinical samples, and to detect mutations in ERG11 gene, and their relationship to fluconazole resistance. Forty-eight isolates of Candida albicans were tested for susceptibility using the disc diffusion method (M-44). ERG11 genes of six isolates were amplif...

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Context 1
... The amplified product in our study was extended from 332 to 1664 bp of the gene, since the hot spot regions of ERG11P located at 105-165, 266-287, and 405-488 [14]. The isolates (3,10) were isolated from different patients, but sharing the same mutations with nine silent (T462C, C558T, C805T, T1143C, A1173G, C1257T, T1350C, C1443T, and T1449G) including two missense mutations (A945C, and G1609A), and four silent mutations (A945C, G1609A, C1443T, and T1449C) detected only in these susceptible isolates (Table 5). These data can be an evidence that these two isolates are belonging to the same strain. ...