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Diet composition of dry and fresh Holstein cows (%). The used total mixed ration (TMR) complied with the Nutrient Requirements of Dairy Cattle recommendations (NRC 2001).
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The transition period is the most critical period in the lactation cycle of dairy cattle. During this period, cows can be exposed to high oxidative stress (OS), which can be managed through mineral supplementation with antioxidant micronutrients. The aim of this study was to evaluate the gene expression profiles of transition dairy cows supplemente...
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Context 1
... cows were fed a total mixed ration (TMR) ad libitum. Nutritional parameters and feed intake of dairy cows complied with the Nutrient Requirements of Dairy Cattle recommendations (Table 1 and 2; NRC 2001). Twelve Holstein cows meeting the following inclusion criteria were selected parity (2 to 5), not receiving parenteral supplementation with minerals 90 days before calving, and not receiving pharmacological treatment 60 days before calving. ...Context 2
... significance was set at p≤0.05, and the power of the t-test was estimated using R studio package STATS. Table S1. Identification of DEG was carried out using the Benjamini and Hochberg method implemented in the DESeq2 software in R ( Love et al. 2014). ...Context 3
... along the sampling period (0 to +14 days) of the significance variables are shown in Figure 2. These absence of significance values need to be validated using higher sampling size according to the t-test power analysis (Table S1). Green and orange dots are represented by supplemented animals and control groups, respectively. ...Context 4
... the current results suggest that supplementation of transition dairy cows with trace minerals would not induce additional changes in the gene expression profiles of these pathways, this could be due to an already elevated expression in response to the high levels of OS and the metabolic and dietary changes associated with this period. Additional supplementation of minerals in dairy cows, which are fed according to NRC guidelines meaning basic mineral requirements are satisfied (As shown in Table 1 and 2), may not be necessary for an increased gene expression response. However, higher availability of these minerals in SG increased AC after calving as shown in Figure 2a, which could be partly explained by an improvement in antioxidant enzyme activity levels because they are necessary cofactors, although differences were not significant ( Ma et al. 2017). ...