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Developmental effects (a, b) and changes in heart beat rate (c–f) after exposition to metandienone. a Regular heart development of medaka embryo after 8 days of development in frontal view (top); after exposition to 50 µM MD, the heart has a tube-like morphology and contractile structures are nearly absent (bottom). b Lateral views as in a. Dotted lines either mark the ventricle or the tube-like heart. Scale bar: 200 µM. (c) Increased heart frequency after exposition to 10 µM MD for 8 days. d–f Increased heart frequency after exposition to 10 µM MD for 2 days in three repeated experiments. Significance code of two-tailed Student’s t test (unpaired): *p value < 0.05; **p value < 0.01; ***p value < 0.001

Developmental effects (a, b) and changes in heart beat rate (c–f) after exposition to metandienone. a Regular heart development of medaka embryo after 8 days of development in frontal view (top); after exposition to 50 µM MD, the heart has a tube-like morphology and contractile structures are nearly absent (bottom). b Lateral views as in a. Dotted lines either mark the ventricle or the tube-like heart. Scale bar: 200 µM. (c) Increased heart frequency after exposition to 10 µM MD for 8 days. d–f Increased heart frequency after exposition to 10 µM MD for 2 days in three repeated experiments. Significance code of two-tailed Student’s t test (unpaired): *p value < 0.05; **p value < 0.01; ***p value < 0.001

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