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Details of studies comparing the application of different BPs (ASV-based vs. OTU-based) to animal origin foodstuff authentication.
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Next Generation Sequencing Technologies (NGS), particularly metabarcoding, are valuable tools for authenticating foodstuffs and detecting eventual fraudulent practices such as species substitution. This technique, mostly used for the analysis of prokaryotes in several environments (including food), is in fact increasingly applied to identify eukary...
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Context 1
... the best of our knowledge, only three studies compared BPs in the analyzing of data from animal foodstuff authentication [25][26][27], likely due to the fact that the application of metabarcoding to this purpose is still rather limited [2] (Table 2). Denay et al. ( [27] and Kappel et al. [25] compared qualitatively and/or quantitatively OTU-based and ASV-based BPs in the analysis of meat samples (Table 2); Klapper et al. [26] compared the results of the sample compositions in canned tuna of six different BPs (OTU-based and ASV-based) ( Table 2). ...Context 2
... the best of our knowledge, only three studies compared BPs in the analyzing of data from animal foodstuff authentication [25][26][27], likely due to the fact that the application of metabarcoding to this purpose is still rather limited [2] (Table 2). Denay et al. ( [27] and Kappel et al. [25] compared qualitatively and/or quantitatively OTU-based and ASV-based BPs in the analysis of meat samples (Table 2); Klapper et al. [26] compared the results of the sample compositions in canned tuna of six different BPs (OTU-based and ASV-based) ( Table 2). According to Denay et al. [27] and Kappel et al. [25], analyses performed using ASVbased (particularly the DADA2 algorithm) BPs presented advantages with respect to BPs applying OTU clustering. ...Context 3
... the best of our knowledge, only three studies compared BPs in the analyzing of data from animal foodstuff authentication [25][26][27], likely due to the fact that the application of metabarcoding to this purpose is still rather limited [2] (Table 2). Denay et al. ( [27] and Kappel et al. [25] compared qualitatively and/or quantitatively OTU-based and ASV-based BPs in the analysis of meat samples (Table 2); Klapper et al. [26] compared the results of the sample compositions in canned tuna of six different BPs (OTU-based and ASV-based) ( Table 2). According to Denay et al. [27] and Kappel et al. [25], analyses performed using ASVbased (particularly the DADA2 algorithm) BPs presented advantages with respect to BPs applying OTU clustering. ...Citations
... 1. The student is performing the first experiments in the wet lab, or analyzes or modeling in silico if the project is in the dry lab (mainly computer-based) [5]. Ideally, there are three stages. ...
Working as a researcher, associate professor, or full professor in the field of biomedicine in Norway involves supervising students preparing for various degrees, such as Bachelor's, Master's, PhD, and MD. Additionally, there are visiting students from other departments and institutions in Norway, as well as from abroad, including various types of exchange students. Departments and Faculties provide guidelines and regulations on what is expected from students and their communication with supervisors throughout the study course. However, these guidelines are often too general, allowing for significant flexibility and varied interpretations from both the students' and supervisors' perspectives. This article focuses on personal experiences supervising biomedical Master's students in Norway. It covers several aspects, including student recruitment, initial instructions, routine work, graduation, and post-graduation interaction. The focus is on the supervisor's point of view, while the students' perspectives on this topic are reserved for further research.
