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Degradation of tributyrin by gastric lipase diffusing from transgenic root powder on an agarose plate. (a) after 1 h; (b) after 4 h; (c) after 24 h. In each plate: left, untransformed root powder; right, transgenic root powder.
Source publication
The properties of recombinant human gastric lipase produced in Arabidopsis thaliana roots have been investigated with the goal of determining the potential of the enzyme. This enzyme is stably bound to roots and can be extracted using a buffer at pH 2.2. This enzyme retains over 75% of its activity after two weeks at room temperature when stored in...
Contexts in source publication
Context 1
... root powder was used as a control. The formation of a clear halo around each grain of transgenic root indicated tributyrin hydrolysis, with the size of the halos increasing with time ( Figure 5). ...
Context 2
... was hydrolyzed around root powder specked on an agarose plate ( Figure 5), which indicated that some gastric lipase was released from the roots and diffused onto the agarose to hydrolyze the substrate. The slow release of lipase at pH 5 initiated the production of butyric acid, which decreased the pH around the roots, causing the release of more enzymes. ...
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Citations
... Apart from the extensive body of research dedicated to exploring bioactive small molecules derived from plants, a notable study by Dr. Guerineau reported the successful production of recombinant human gastric lipase by Arabidopsis thaliana root culture while also providing a comprehensive characterization of the enzyme's properties [13]. This investigation showcases the application of molecular pharming in plants as a means to synthesize recombinant proteins, which are big molecules. ...
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